Isolation of basidiomycetous yeast Pseudozyma tsukubaensis and production of glycolipid biosurfactant, a diastereomer type of mannosylerythritol lipid-B

Applied Microbiology and Biotechnology
Tomotake MoritaDai Kitamoto

Abstract

The producers of glycolipid biosurfactant, mannosylerythritol lipid-B (MEL-B), were isolated from leaves of Perilla frutescens on Ibaraki in Japan. Four isolates, 1D9, 1D10, 1D11, and 1E5, were identified as basidiomycetous yeast Pseudozyma tsukubaensis by rDNA sequence and biochemical properties. The structure of MEL-B produced by these strains was analyzed by (1)H nuclear magnetic resonance and gas chromatography-mass spectrometry methods, and was determined to be the same as the diastereomer MEL-B produced by P. tsukubaensis NBRC 1940. Of these isolates, P. tsukubaensis 1E5 (JCM 16987) is capable of producing the largest amount of the diastereomer MEL-B from vegetable oils. In order to progress the diastereomer MEL-B production by strain 1E5, factors affecting the production, such as carbon and organic nutrient sources, were further examined. Olive oil and yeast extract were the best carbon and nutrient sources, respectively. Under the optimal conditions, a maximum yield, productivity, and yield coefficient of 73.1 g/L, 10.4 g L(-1) day(-1), and 43.5 g/g were achieved by feeding of olive oil in a 5-L jar-fermenter culture using strain 1E5.

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May 1, 2010·Journal of Oleo Science·Tomotake MoritaDai Kitamoto

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Citations

Apr 16, 2013·Applied Microbiology and Biotechnology·Tomotake MoritaDai Kitamoto
Mar 4, 2014·Journal of Bioscience and Bioengineering·Takashi WatanabeHiroko Kuze Kitamoto
Dec 30, 2015·FEMS Yeast Research·Antonio Adalberto Kaupert NetoJuliana Velasco de Castro Oliveira
Mar 10, 2015·Journal of Oleo Science·Tomotake MoritaDai Kitamoto
Apr 1, 2014·International Journal of Systematic and Evolutionary Microbiology·Juliana Velasco de Castro OliveiraDiego Mauricio Riaño-Pachón
Dec 17, 2016·Genome Announcements·Elena GeiserLars M Blank
Oct 27, 2017·FEBS Letters·Sylwia JezierskaInge Van Bogaert
May 11, 2015·Applied Microbiology and Biotechnology·Tomotake MoritaDai Kitamoto
Aug 8, 2018·World Journal of Microbiology & Biotechnology·Sineenath KunthiphunAncharida Savarajara
Jun 22, 2018·Applied Microbiology and Biotechnology·Azusa SaikaTomotake Morita
Nov 16, 2016·Applied Microbiology and Biotechnology·Bruno Nicolau PaulinoGlaucia Maria Pastore
Nov 17, 2019·Journal of Bioscience and Bioengineering·Azusa SaikaTomotake Morita
Apr 17, 2021·Biotechnology for Biofuels·Shahryar ShakeriMahmood Maleki
Apr 17, 2021·Essays in Biochemistry·Nick WierckxKerstin Schipper

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