Isolation of bovine lactoferrin, lactoperoxidase and enzymatically prepared lactoferricin from proteolytic digestion of bovine lactoferrin using adsorptive membrane chromatography

Journal of Chromatography. a
Kerstin PlateThomas Scheper

Abstract

A new downstream procedure for the isolation of bovine lactoferrin (bLf), lactoperoxidase and bovine lactoferricin (LfcinB) from sweet cheese whey was developed at the laboratory scale, based on membrane adsorber technology. The procedure was upscaled later on to an industrially relevant scale for the purificationof sweet whey concentrate with a recovery yield for lactoferrin of more than 90%. Based on these results the industrial process for 1 x 10(8) kg whey per year was projected. These high-value proteins were downstreamed by using cation-exchange membrane systems (Sartobind S, Sartorius, Göttingen, Germany). These strongly acidic membranes trap proteins in its anionic form. The dynamic loading capacity for both proteins as well as the optimal elution profiles with sodium chloride gradients were derived from laboratory experiments using membrane modules with 15-75 cm2 membrane material. Further investigations were performed with 1 m2 modules in a continuous process mode. The enzymatic preparation of LfcinB from bLf was performed by pepsin hydrolysis and the isolation of LfcinB was directly carried out from the enzymatic digest mixture. The identification of the proteins was performed with matrix-assisted laser desorption io...Continue Reading

Citations

Feb 9, 2012·Journal of Agricultural and Food Chemistry·Andreas Juadjur, Peter Winterhalter
Dec 19, 2008·Critical Reviews in Food Science and Nutrition·Lígia RodriguesHelena Lindmark Månsson
Jan 30, 2013·Biotechnology Advances·Valerie OrrC Perry Chou
May 1, 2009·Biotechnology and Bioengineering·Jagan M Billakanti, Conan J Fee
Feb 10, 2009·Biotechnology and Bioengineering·Syed M Saufi, Conan J Fee
Sep 24, 2014·Colloids and Surfaces. B, Biointerfaces·Junfen Sun, Lishun Wu
Oct 21, 2017·Journal of Food Science and Technology·Swapnali S PawarPrasanna D Belur
Jan 1, 2014·Journal of Separation Science·María Fernanda BaieliFederico Javier Wolman
Apr 22, 2019·Journal of Dairy Science·Zeineb JradNadia Oulahal
Nov 16, 2016·The Journal of Dairy Research·Catherine JeanGilles Robitaille
Jul 25, 2020·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Bo LönnerdalRulan Jiang
Oct 6, 2009·Journal of Chromatography. a·Brian D BowesAbraham M Lenhoff

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