Isolation of Breast cancer CTCs with multitargeted buoyant immunomicrobubbles.

Colloids and Surfaces. B, Biointerfaces
Guankui WangDmitri Simberg

Abstract

Circulating tumor cells (CTCs) are extremely rare cells found in blood of metastatic cancer patients. There is a need for inexpensive technologies for fast enrichment of CTCs from large blood volumes. Previous data showed that antibody-conjugated lipid shell immuno-microbubbles (MBs) bind and isolate cells from biological fluids by flotation. Here, blood-stable MBs targeted to several surface markers for isolation of breast tumor cells were developed. MBs coated with anti-human EpCAM antibodies showed efficient binding of EpCAM+ breast cancer cell lines SKBR-3, MCF-7, and MDA-MB-453, whereas anti-human EGFR MBs showed binding of EpCAMLOW/NEGATIVE cell lines MDA-MB-231 and BT-549. Multitargeted anti-human EpCAM/EGFR MBs bound all cell lines with over 95% efficiency. Highly concentrated MB-bound tumor cells were collected in a microliter volume via an inverted vacuum-assisted harvesting setup. Using anti-EpCAM and/or anti-EpCAM/EGFR MBs, an efficient (70-90%) recovery and fast (30min) isolation of the above-mentioned cells and cell clusters was achieved from 7.5mL of spiked human blood. Using anti-EpCAM MBs and anti-EpCAM/EGFR MBs, cytokeratin-positive, CD45-negative CTCs were detected in 62.5% (10/16) of patients with metastatic...Continue Reading

References

Jan 1, 1989·Ultrasound in Medicine & Biology·J Ophir, K J Parker
Aug 8, 1998·Ultrasound in Medicine & Biology·A KabalnovJ Weers
Feb 15, 2001·Echocardiography·Helene Von BibraAnders Juhlin-Dannfeldt
Feb 18, 2004·Neoplasia : an International Journal for Oncology Research·Harry H RuanW Steve Ammons
Jun 2, 2004·Nature Reviews. Cancer·Klaus Pantel, Ruud H Brakenhoff
Aug 20, 2004·The New England Journal of Medicine·Massimo CristofanilliDaniel F Hayes
Oct 27, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·W Jeffrey AllardLeon W M M Terstappen
Oct 26, 2005·Theriogenology·Duane L Garner
Aug 8, 2006·Nature Medicine·Patricia S Steeg
Oct 24, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Lyndsay HarrisUNKNOWN American Society of Clinical Oncology
Jun 10, 2009·Journal of Drug Targeting·Dmitri Simberg, Robert Mattrey
Feb 12, 2010·Annals of Oncology : Official Journal of the European Society for Medical Oncology·F A W CoumansL W M M Terstappen
Sep 4, 2010·Breast Cancer Research : BCR·Aleix PratCharles M Perou
Oct 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shannon L StottMehmet Toner
Dec 15, 2010·Ultrasound in Medicine & Biology·Astrid Hilde MyrsetRoald Skurtveit
Feb 2, 2011·BMC Cancer·Johanna M GostnerGilbert Spizzo
Feb 3, 2011·Nature Communications·Leonora BalajJohan Skog
Feb 9, 2011·The Journal of Cell Biology·Min YuDaniel A Haber
Mar 23, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Max S Wicha, Daniel F Hayes
May 18, 2011·Journal of Oncology·Stephen D MikolajczykTony J Pircher
Jun 15, 2011·Molecular Cancer Research : MCR·Andrew J ArmstrongMariano A Garcia-Blanco
Apr 12, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Elizabeth A PunnooseMark R Lackner
Sep 11, 2012·Advances in Colloid and Interface Science·James J Kwan, Mark A Borden
Aug 27, 2013·Methods : a Companion to Methods in Enzymology·Guixin ShiDmitri Simberg
Mar 8, 2014·Breast Cancer Research : BCR·Maryam B LustbergJeffrey J Chalmers
Mar 22, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·P K GroverJ E Hardingham
Nov 16, 2014·EMBO Molecular Medicine·Simon A JoosseKlaus Pantel
Jan 17, 2015·Cancer Research·Laure CayrefourcqCatherine Alix-Panabières
Jul 18, 2015·Scientific Reports·Sanne de WitLeon W M M Terstappen
Nov 4, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michail IgnatiadisStefanie S Jeffrey
Dec 4, 2015·Scientific Reports·Monika VishnoiDario Marchetti
Dec 10, 2015·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·R Ramos-MedinaM Martin

❮ Previous
Next ❯

Citations

Mar 21, 2020·International Journal of Molecular Sciences·Ivan PrestaGiuseppe Donato
Jun 14, 2021·Advances in Colloid and Interface Science·Marie Pierre Krafft, Jean G Riess
Aug 10, 2021·ACS Chemical Biology·Ruey-Herng LeeChi-Huey Wong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Imaging

Imaging techniques, including CT and MR, have become essential to tumor detection, diagnosis, and monitoring. Here is the latest research on cancer imaging.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.