Isolation of cancer stem-like cells from a side population of a human glioblastoma cell line, SK-MG-1

Cancer Letters
Raita FukayaMasahiro Toda

Abstract

Accumulating evidence suggests that in several types of brain tumors, including glioma, only a phenotypic subset of tumor cells called brain cancer stem cells (BCSCs) may be capable of initiating tumor growth. Recently, the isolation of side population (SP) cells using Hoechst dye has become a useful method for obtaining cancer stem cells in various tumors. In this study, we isolated cancer stem-like cells from human glioma cell lines using the SP technique. Flow cytometry analysis revealed that SK-MG-1, a human glioblastoma cell line, contained the largest number of SP cells among the five glioma cell lines that were analyzed. The SP cells had a self-renewal ability and were capable of forming spheres in a neurosphere culture medium containing EGF and FGF2. Spheres derived from the SP cells differentiated into three different lineage cells: neurons, astrocytes and oligodendrocytes. RT-PCR analysis revealed that the SP cells expressed a neural stem cell marker, Nestin. The SP cells generated tumors in the brains of NOD/SCID mice at 8weeks after implantation, whereas the non-SP cells did not generate any tumors in the brain. These results indicate that SP cells isolated from SK-MG-1 possess the properties of cancer stem cells, i...Continue Reading

References

Apr 1, 1996·The Journal of Experimental Medicine·M A GoodellR C Mulligan
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·L A DoyleD D Ross
Jun 8, 2001·Genes & Development·E A MaherR A DePinho
Aug 3, 2002·Nature Reviews. Cancer·Yuan Zhu, Luis F Parada
Oct 23, 2003·Nature Reviews. Cancer·Freddy Radtke, Kenneth Raj
Dec 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·Houman D HemmatiHarley I Kornblum
Jan 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Toru KondoTetsuya Taga
Apr 28, 2004·Current Opinion in Genetics & Development·Andrew P Weng, Jon C Aster
Sep 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·C Hirschmann-JaxM K Brenner
Nov 19, 2004·Nature·Sheila K SinghPeter B Dirks
Nov 24, 2004·Oncogene·Xiangpeng YuanJohn S Yu
Apr 2, 2005·Nature Reviews. Cancer·Michael DeanSusan Bates
May 27, 2005·Nature Neuroscience·Keejung Yoon, Nicholas Gaiano
Aug 27, 2005·The New England Journal of Medicine·Nader SanaiMitchel S Berger
Apr 25, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Toru Kondo
May 26, 2006·Nature Reviews. Cancer·Angelo L VescoviBrent A Reynolds
Jan 16, 2007·Neoplasia : an International Journal for Oncology Research·Alan H Shih, Eric C Holland
Jan 16, 2007·Cancer Cell·Christopher CalabreseRichard J Gilbertson
Feb 21, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Peter B Dirks
Apr 19, 2007·Cancer Research·Xuesheng ZhengWeiguo Liu
Jul 20, 2007·Journal of Cell Science·Sumitra BhattacharyaIqbal Ahmad
Aug 10, 2007·Cellular and Molecular Life Sciences : CMLS·U Koch, F Radtke

❮ Previous
Next ❯

Citations

Dec 17, 2011·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Zhifa ZhangRenyi Qin
Oct 18, 2012·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yatong ChenYongguang Jiang
May 11, 2012·Frontiers of Medicine·Douglas D FangYajun Xu
May 24, 2011·Molecular Cancer·Masanao TabuseMasahiro Toda
May 13, 2010·Cell Communication and Signaling : CCS·Yvonne WelteChristian Ra Regenbrecht
Dec 3, 2013·BioMed Research International·Vinitha RichardM Radhakrishna Pillai
Jan 28, 2014·Journal of Molecular Neuroscience : MN·Daming ZhangShiguang Zhao
May 14, 2014·International Journal of Molecular Sciences·Marius Eugen CiureaAnica Dricu
Oct 16, 2014·Cancer Cell International·Susan Ling Ling HoeAlan Soo Beng Khoo
Jul 3, 2015·Stem Cells International·Katrin LiffersAlexander Schulte
Apr 18, 2015·Journal of Hematology & Oncology·Axel SchulenburgPeter Valent
Mar 20, 2015·Molecular Biology of the Cell·Wei ZhaoYi-Xue Xue
May 20, 2011·International Journal of Radiation Oncology, Biology, Physics·James C MarshRichard W Byrne
Mar 6, 2012·Biochemical and Biophysical Research Communications·Xin-xing LiJing Yi
Apr 3, 2012·World Journal of Gastrointestinal Oncology·Alba RoccoGerardo Nardone
Oct 5, 2010·International Journal of Cancer. Journal International Du Cancer·Jérôme KroonenBernard Rogister
Jul 6, 2014·International Journal of Oncology·Viviana CastilloEnrique A Castellon
Jan 11, 2013·Cancer Control : Journal of the Moffitt Cancer Center·James C MarshAidnag Z Diaz
May 31, 2017·Journal of Neuro-oncology·Ryogo KikuchiMasahiro Toda

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Cancer Stem Cells in Glioblastoma

Glioblastoma is the most common and aggressive type of brain tumor. It contains a population of tumor initiating stem cell-like cells known as cancer stem cells. Investigations are ongoing into these cancer stem cells found in these solid tumors which are highly resistance to treatment. Here is the latest research on cancer stem cells in glioblastoma.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.