Isolation of candidate cDNAs for portions of the Duchenne muscular dystrophy gene

Nature
A P MonacoL M Kunkel

Abstract

Duchenne muscular dystrophy (DMD) and the less severe Becker muscular dystrophy (BMD) are human X-linked muscle-wasting disorders that have been localized to the band Xp21 by genetic linkage analysis and cytologically detectable abnormalities. A cloned DNA segment, DXS164 (or pERT87), has been shown to detect deletions in the DNA of unrelated DMD and BMD males. Here we present the nucleotide sequence of two highly conserved DNA fragments from the DXS164 locus and their homologous sequences from the mouse X chromosome. One of the human conserved segments hybridized to a large transcript in RNA isolated from human fetal skeletal muscle and was used to isolate cDNA clones which cover approximately 10% of this transcript. The cDNA clones map to Xp21 and hybridize with a minimum of eight small regions that span 130 kilobases (kb) of the DXS164 locus. These expressed sequences are candidates for portions of the gene responsible for both DMD and BMD.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L M KunkelS A Latt
Jan 1, 1985·Cytogenetics and Cell Genetics·P N GoodfellowH H Ropers
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A M MichelsonS H Orkin
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E B Keller, W A Noon
Nov 15, 1983·Journal of Molecular Biology·A M FrischaufN Murray
Nov 18, 1983·Science·R A Young, R W Davis
Jan 22, 1982·Nucleic Acids Research·S M Mount

❮ Previous
Next ❯

Citations

Feb 19, 2000·Microscopy Research and Technique·A A HackE M McNally
Apr 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·W E TidymanE Bandman
May 8, 2000·Medicinal Research Reviews·D R Bentley
Dec 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J SchofieldY H Edwards
Dec 1, 1993·Annals of Neurology·J B Martin
Mar 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·U Hochgeschwender, M B Brennan
Aug 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C M Milner, R D Campbell
Dec 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B Holdener-KennyT Magnuson
Jan 1, 1994·Journal of Magnetic Resonance Imaging : JMRI·Y HuangR G Miller
Jan 1, 1990·Muscle & Nerve·S HollingworthE Robson
Jun 1, 1990·Muscle & Nerve·C G Carlson, R V Makiejus
Feb 1, 1991·Muscle & Nerve·M MiyatakeG Usuku
May 1, 1992·Muscle & Nerve·J HuardJ P Tremblay
Oct 1, 1992·Muscle & Nerve·E E Dupont-Versteegden, R J McCarter
Mar 1, 1989·Human Genetics·S Liechti-GallatiH Moser
Mar 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J F CoyA Poustka
Apr 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A J BrookesD J Porteous
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L Stubbs
Sep 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M A NorthH Lehrach
May 1, 1988·European Journal of Pediatrics·G RomeoG Galluzzi
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M L KlebigE M Rinchik
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M K MaconochieA J Greenfield
Apr 1, 1989·Journal of Bioenergetics and Biomembranes·R A Sabbadini, A S Dahms
Mar 1, 1993·Behavior Genetics·E J Devor
Mar 1, 1990·Behavior Genetics·S HodgkinsonH M Gurling
Jul 1, 1990·Somatic Cell and Molecular Genetics·T V StrongS J Anderson
Nov 1, 1987·Somatic Cell and Molecular Genetics·J S ChamberlainV M Chapman
Jan 1, 1988·Journal of Inherited Metabolic Disease·M ShimmotoY Suzuki
Jan 1, 1991·European Archives of Psychiatry and Clinical Neuroscience·M J Owen, P McGuffin
May 1, 1990·Indian Journal of Pediatrics·L Specht
Jan 23, 1998·Molecular Biotechnology·S Parimoo
Jan 1, 1987·Molecular Neurobiology·X O Breakefield, A I Geller

❮ Previous
Next ❯

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