Isolation of cardiac mast cells in experimental Trypanosoma cruzi infection

Tissue & Cell
Marcelo Meuser-BatistaAndrea Henriques-Pons

Abstract

Mast cells (MC) secrete diverse pre-stored chemical mediators that are pivotal in inflammatory and fibrotic etiologies, such as Trypanosoma cruzi-induced myocardiopathy. However, due to reduced number of cardiac MC, in situ and in vitro identification, and difficult tissue isolation, these cells are rarely addressed. In this work we optimized the identification of cardiac and peritoneal MC and developed an enzymatic method for MC isolation using control and T. cruzi-infected mice. MC were identified by: toluidine blue (TB); alcian blue (AB)/safranin (S); AB or a mixed solution composed by AB/S/TB. Previous evaluations of cardiac MC in T. cruzi infection were based on TB staining and our results using AB/S/TB solution showed an increase in, at least, five times the detection of MC. This mixed solution may improve the identification of MC populations also from skin, mucosa and tissues that are infected by other pathogens or under the influence of chronic inflammation, leading to more precise results. Furthermore, the appropriate combination of samples (frozen/unfixed/thick slices) and staining protocols can assure the best evaluation of MC. We have also isolated cardiac MC using collagenase and developed a highly efficient 60%/70...Continue Reading

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Citations

Feb 2, 2012·Cardiology in Review·Fabiana S MachadoHerbert B Tanowitz
Jan 29, 2016·The Journal of Pharmacy and Pharmacology·Eroneide S B CardosoSara M Thomazzi
Aug 9, 2011·The American Journal of Pathology·Marcelo Meuser-BatistaAndrea Henriques-Pons
Nov 18, 2015·Scientific Reports·Thaise Lara TeixeiraClaudio Vieira da Silva
Apr 7, 2015·Journal of Ethnopharmacology·Polyana B F DinizSara M Thomazzi
Jul 14, 2020·Frontiers in Immunology·Flávia Alves MartinsClaudio Vieira da Silva
Nov 23, 2017·Frontiers in Cellular and Infection Microbiology·Aline A da SilvaClaudio V da Silva

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