Isolation of catalase-negative Listeria monocytogenes strains from listeriosis patients and their rapid identification by anti-p60 antibodies and/or PCR.

Journal of Clinical Microbiology
A BubertP Schubert

Abstract

Two catalase-negative Listeria monocytogenes serovar 1/2b strains were isolated from listeriosis patients in 1995 in Germany. The infections appeared in individuals from different cities at different seasons and were caused by L. monocytogenes strains of different clonal types. In particular, the catalase reaction of one strain isolated from blood was consistently negative, whereas this reaction was only reversibly blocked when the strain was freshly isolated from ascitic fluid. After subculturing, the catalase-positive reaction was restored. Initially, identification of these isolates was difficult to achieve not only because of the lack of a catalase reaction, which generally distinguishes L. monocytogenes from other morphologically similar pathogenic gram-positive bacteria, but also because other routinely used biochemical tests such as CAMP and the commercial API test gave unclear results. However, rapid and unequivocal identification of these strains was possible by analyzing secretions of the p60 protein in culture supernatants by enzyme-linked immunosorbent assay or Western blot (immunoblot) analysis with our recently developed Listeria- and L. monocytogenes-specific anti-p60 antibodies. Additionally, the identifications...Continue Reading

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Citations

Apr 26, 2000·FEMS Microbiology Letters·J H ParkB S Yoon
Jul 4, 2001·Clinical Microbiology Reviews·J A Vázquez-BolandJ Kreft
Nov 9, 2000·Letters in Applied Microbiology·N J RowanA A Candlish
May 13, 2004·Clinical and Diagnostic Laboratory Immunology·Kang-Y YuByung-S Youn
Sep 8, 2010·Applied and Environmental Microbiology·Reha Onur Azizoglu, Sophia Kathariou
Jul 28, 2012·Scandinavian Journal of Gastroenterology·George D LiatsosAntonios Moulakakis
Dec 5, 2012·The Journal of Veterinary Medical Science·Yoshimasa SasakiYukiko Yamada
Oct 3, 1999·Applied and Environmental Microbiology·A BubertM Wagner
May 5, 2006·Journal of Clinical Microbiology·J A CepedaD W Wareham
Apr 29, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A von Graevenitz

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