PMID: 9431680Jan 1, 1997Paper

Isolation of cDNA clones for genes showing enhanced expression in barley leaves during dark-induced senescence as well as during senescence under field conditions

Planta
T Kleber-Janke, K Krupinska

Abstract

Senescence of barley (Hordeum vulgare L. cv. Carina) primary foliage leaves was induced by transfer of the plants into darkness for 2 d. Under these conditions senescence was characterized by a light-reversible decline in the efficiency of photosystem II, and in chlorophyll and protein contents. To isolate senescence-associated genes a differential display of cDNA fragments amplified from reversely transcribed RNA was employed. By this method, gene expression in leaves of control plants collected at the onset of the dark period was compared with gene expression in senescing leaves collected at the end of the extended dark period. The expression of the genes represented by various differentially displayed cDNA fragments was examined by Northern blot hybridizations with RNA derived from primary foliage leaves before and after induction of senescence by darkness. In order to test whether these genes with enhanced expression during dark-induced senescence also show enhanced expression during natural senescence, Northern blot hybridizations were carried out with RNA samples prepared from flag leaves of barley plants during maturation and senescence under field conditions. Five of the cDNA fragments representing transcripts associate...Continue Reading

Citations

Jul 31, 2012·Planta·Sarah M PilkingtonAndrew C Allan
Oct 16, 2012·Plant Molecular Biology·Yongfeng Guo
Sep 21, 2000·Environmental and Experimental Botany·A FangmeierK Krupinska
Sep 30, 2000·Plant Science : an International Journal of Experimental Plant Biology·J WeiA L Plant
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·T Kleber-JankeW M Becker
Dec 7, 2006·Plant Biotechnology Journal·Vicky Buchanan-WollastonDavid Pink
Jun 13, 2002·Physiologia Plantarum·Yih-Jong HuangShu-Chen Grace Chen
Mar 7, 2014·Journal of Experimental Botany·Mercedes Díaz-MendozaIsabel Díaz
Feb 23, 2010·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Wenyi YanXiaoming Zhang
Oct 12, 2015·Journal of Plant Physiology·Gregorio Gálvez-ValdiviesoMiguel Aguilar
Aug 15, 2015·Journal of Experimental Botany·Jing WangCordelia Bolle
Sep 20, 2008·Plant Biology·P L GregersenK Krupinska
Sep 1, 2006·Plant Science : an International Journal of Experimental Plant Biology·David P Dixon, Robert Edwards
Mar 17, 2004·Journal of Plant Physiology·Christophe FargeixFrançois Widmer
Jul 13, 2005·Journal of Plant Physiology·Dean DellaPenna
Feb 1, 2015·Journal of Plant Physiology·Armin SpringerChristiane Reinbothe
Jan 1, 2013·Antioxidants·Marina CavaiuoloAntonio Ferrante
Oct 17, 2017·International Journal of Molecular Sciences·Muhammad JehanzebYing Miao
Nov 5, 2015·BMC Plant Biology·Xiaoying HuoXunyan Liu
Mar 16, 2019·Genes·Aleksandra Swida-Barteczka, Zofia Szweykowska-Kulinska
Dec 16, 2019·Applied Microbiology and Biotechnology·Panhong YuanLong Liu
Jun 29, 2004·Molecular Genetics and Genomics : MGG·T T T NguyenH T Nguyen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.