PMID: 6412232Sep 1, 1983Paper

Isolation of cDNA clones for the p33 invariant chain associated with HLA-DR antigens

Proceedings of the National Academy of Sciences of the United States of America
E O LongB Mach

Abstract

HLA-DR antigens are polymorphic cell surface glycoproteins involved in the control of the immune response in man. They consist of two subunits, the alpha and the beta chains. In addition, an invariant glycoprotein of Mr 33,000 (DRp33) is associated intracellularly with HLA-DR antigens. A cDNA clone for DRp33, called 33-10, was isolated. Because no amino acid sequence has yet been determined for DRp33 the identification of cDNA clone 33-10 was based on selection of mRNA by hybridization, subsequent translation in a rabbit reticulocyte lysate supplemented with microsomes, and translation in microinjected Xenopus oocytes followed by immunoprecipitation with an anti-DR antiserum. The translation products assembled with DR alpha and beta chains in oocytes coinjected with all three mRNAs. Assembly of DR alpha and beta chains was also observed in the absence of DRp33 mRNA. Furthermore, when compared with DRp33 immunoprecipitated from a human B-cell line, translation products of the hybrid-selected mRNA showed (i) identical migration in two-dimensional gel electrophoresis, (ii) identical apparent molecular weight in the absence of N-linked glycosylation, and (iii) a very similar two-dimensional peptide map. Transcription of the DRp33 g...Continue Reading

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Citations

Jan 1, 1990·Immunologic Research·P CresswellM S Marks
Aug 29, 2007·Cancer Immunology, Immunotherapy : CII·James A ThompsonSuzanne Ostrand-Rosenberg
Jan 1, 1991·The International Journal of Biochemistry·H C WangM R Ziai
May 1, 1986·Molecular Immunology·C Kinnon, M J Owen
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D NizetićJ Klein
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C R HumeJ S Lee
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C DoyleJ L Strominger
Nov 1, 1986·The Journal of Experimental Medicine·J Miller, R N Germain
Dec 9, 2009·Expert Opinion on Investigational Drugs·Zuzana BerkovaFelipe Samaniego
Oct 15, 2011·Developmental and Comparative Immunology·Michael F CriscitielloMartin F Flajnik
Oct 12, 2014·Journal of Experimental & Clinical Cancer Research : CR·Zuzana BerkovaFelipe Samaniego
Mar 20, 2013·European Journal of Haematology·Alessandro AllegraCaterina Musolino
Apr 2, 2016·Biochimica Et Biophysica Acta·Bernd Schröder
May 21, 2016·Circulation Research·Lukasz PrzybylFlorian Herse
Oct 25, 2017·Current Hypertension Reports·Ross McNallyLana McClements
Feb 21, 2002·Biochimica Et Biophysica Acta·Pamela Stumptner-Cuvelette, Philippe Benaroch

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