Isolation of cDNAs representing dithiolethione-responsive genes

Carcinogenesis
T PrimianoT R Sutter

Abstract

Dithiolethiones inhibit tumorigenicity elicited by many structurally diverse carcinogens in numerous target tissues. These protective actions are associated with the induction of several carcinogen detoxification enzymes, some of which have only recently been discovered. In order to identify additional novel inducible detoxification response genes, a cDNA library was prepared from liver of rats treated with 1,2-dithiole-3-thione (D3T) and was screened by a differential hybridization method. Complementary DNA clones for several known D3T-inducible genes were isolated, such as epoxide hydrolase, aflatoxin B1-aldehyde reductase, quinone reductase and multiple subunits of glutathione S-transferase. Clones representing genes not previously associated with detoxification were isolated, including those for ferritin heavy and light subunits, ribosomal proteins L18a and S16 and two novel genes, termed dithiolethione-inducible genes (or DIG-1 and DIG-2). Levels of mRNA recognized by each clone were increased from 2- to 31-fold, with maximum induction between 6 and 30 h after treatment with D3T. Except for epoxide hydrolase, the kinetics of induction of each mRNA was coordinate with increased rates of gene transcription. However, based on...Continue Reading

Citations

May 27, 1999·Electrophoresis·F A WitzmannF L Siegel
May 7, 1999·Archives of Pharmacal Research·N D Kim, S G Kim
Aug 17, 2001·Mutation Research·M K KwakT W Kensler
Feb 19, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T PrimianoT W Kensler
May 10, 2000·Free Radical Biology & Medicine·M A OtienoK Z Guyton
Mar 10, 2007·Carcinogenesis·Brian S CornblattKala Visvanathan
Jun 8, 2001·Alcoholism, Clinical and Experimental Research·C C CunninghamK Torii
May 14, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Matthew D MerrellNathan J Cherrington
Aug 17, 2012·The Journal of Pharmacology and Experimental Therapeutics·Xiang YuShana J Sturla
Feb 24, 2001·Drug Metabolism and Drug Interactions·T W KenslerB D Roebuck
May 23, 2001·Archives of Biochemistry and Biophysics·T YokomizoT Shimizu
Aug 2, 2005·Nutritional Neuroscience·M H Noyan-AshrafB H J Juurlink
Jul 15, 2009·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Yuhua ZhaoHsin-Hsiung Tai
Dec 7, 2007·International Journal of Cancer. Journal International Du Cancer·Jin-Hee LeeSung-Gil Chi
Apr 18, 2008·Journal of Biochemical and Molecular Toxicology·Matthew D MerrellNathan J Cherrington
Feb 1, 2005·Archives of Biochemistry and Biophysics·Murugesan VelayuthamJay L Zweier
Oct 13, 2004·Mutation Research·Mi-Kyoung KwakThomas W Kensler
Aug 29, 2006·Free Radical Biology & Medicine·Bente GlintborgHenrik E Poulsen
Sep 29, 2005·Biochemical and Biophysical Research Communications·Yee Liu ChuaJeffrey S Armstrong
Sep 3, 2014·Journal of Biochemical and Molecular Toxicology·Melanie M ErzingerShana J Sturla
May 24, 2014·The International Journal of Biochemistry & Cell Biology·Ricardo Sánchez-RodríguezJulio Isael Pérez-Carreón
Sep 19, 2012·Atherosclerosis·Bernhard H J Juurlink
Feb 18, 2004·The Journal of Biological Chemistry·Ryan A Dick, Thomas W Kensler
Oct 18, 2002·Expert Review of Anticancer Therapy·Ryan A Dick, Thomas W Kensler
Jul 24, 2013·The Journal of Biological Chemistry·Dario A VitturiFrancisco J Schopfer
Nov 19, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·A Kenneth MacLeodJohn D Hayes
Nov 30, 2019·Archives of Pharmacal Research·Keiko Taguchi, Thomas W Kensler
Oct 27, 2020·Frontiers in Pharmacology·Xin AnHua Zhu
May 27, 2008·Journal of the American Chemical Society·Despina J Bougioukou, Jon D Stewart
Aug 24, 2021·Frontiers in Pharmacology·Xing WangZufeng Guo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.