PMID: 9187304May 17, 1997Paper

Isolation of ceramide fractions from human stratum corneum lipid extracts by high-performance liquid chromatography

Biochimica Et Biophysica Acta
T Gildenast, J Lasch

Abstract

This report describes a procedure to isolate ceramides from human stratum corneum lipid extracts using preparative liquid chromatography (LC) and semi-preparative high-performance liquid chromatography (HPLC) methods, which is economical in construction and operation. The composition of isolated individual 'peaks' of separated ceramide fractions is reported. These peaks were quantified by scanning of the chromatographic plates stained after high performance thin-layer chromatography (HPTLC). The HPTLC was done by automated multiple development (AMD). The enrichment of the ceramides by liquid chromatography turned out to be necessary for their separation from more polar lipids of the human stratum corneum lipid extract. Only the evaporative light scattering detector gave a stable baseline, reproducible results and eliminated the 'solvent front' in which peaks of interest could coelute. Fluorescence measurements of extracted human stratum lipids indicated lipofuscin-like substances.

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Citations

May 8, 2001·Lipids·J P VietzkeU Hintze
Mar 9, 2013·Veterinary Dermatology·Mandy Angelbeck-SchulzeReinhard Mischke
Feb 22, 2012·Animal Science Journal = Nihon Chikusan Gakkaihō·Yuko Haruta-OnoToshimitsu Yoshioka
Oct 30, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Jonathan A PaleroHans C Gerritsen
Feb 11, 2018·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·S-S ChengX-J Zhang
Mar 21, 2006·Journal of Separation Science·Loïc QuintonPierre Chaminade
Mar 25, 2017·Critical Reviews in Analytical Chemistry·Kamil JurowskiBogusław Buszewski
Feb 5, 2003·Journal of Chromatography. a·Minlian ZhangZheng Li
Mar 21, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hany FarwanahKonrad Sandhoff

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