PMID: 6987527Mar 13, 1980Paper

Isolation of chromosomal origins of replication in yeast

Nature
D BeachS Shall

Abstract

Origins of replication have been identified in the DNA of viruses, mitochondria, bacterial plasmids and the bacterial chromosome. However, origins of replication of eukaryote chromosomes have remained elusive because of the large size and sequence complexity of chromosomes and in particular for want of a suitable assay for their detection. Recent development of techniques for genetic transformation of yeast by autonomously replicating cytoplasmic plasmids now makes it possible to search for eukaryote origins in a manner analogous to that used for bacteria. Here we describe the construction and properties of a plasmid which contains no effective eukaryote replication origin and whose efficiency of replication in yeast is greatly enhanced by insertion of certain fragments of yeast chromosomal DNA. We believe these to contain replication origins, since yeast transformants are shown to contain copies of the transforming plasmids.

References

Oct 1, 1978·Nucleic Acids Research·K N Subramanian, T Shenk
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F BolivarH W Boyer
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A HinnenG R Fink
Jul 4, 1978·Molecular & General Genetics : MGG·W MesserK von Meyenburg
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K StruhlR W Davis
Nov 1, 1979·Nature·D T StinchcombR W Davis
Oct 1, 1975·Experimental Cell Research·T D Petes, D H Williamson
Jan 4, 1974·Nature·Carol S NewlonW L Fangman
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D B Clewell, D R Helinski

Citations

Jan 1, 1981·Molecular & General Genetics : MGG·J A GormanN Warren
Sep 1, 1988·Molecular & General Genetics : MGG·A DüvellP Tudzynski
Jul 1, 1983·Current Genetics·C GaillardinH Heslot
Jan 1, 1986·Current Genetics·B Böckelmann, K Esser
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C S Chan, B K Tye
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D T StinchcombR W Davis
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·V A Zakian
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B C HymanR H Rownd
Aug 1, 1994·Molecular Biology of the Cell·J G WohlgemuthM P Calos
Aug 26, 1986·Nucleic Acids Research·J S MillsS M Kingsman
Mar 10, 2010·Nucleic Acids Research·Gwynedd A BendersClyde A Hutchison
Dec 3, 1999·Biochemical and Biophysical Research Communications·S ReppeT B Oyen
Mar 17, 1986·FEBS Letters·M A PeñalvaV Rubio
Jul 1, 1982·Plasmid·V A Zakian, D M Kupfer
Mar 1, 1983·Cell·W L FangmanE Chlebowicz-Sledziewska
Aug 1, 2015·Scientific Reports·Waranya NatesuntornSatoshi Harashima
Oct 5, 1984·Journal of Molecular Biology·J B FeldmanJ R Broach
Feb 14, 1984·Biochemical and Biophysical Research Communications·T R Rao, C A Reddy

Related Concepts

Chromosomes
DNA Replication
DNA, Fungal
Episomes
Saccharomyces cerevisiae
Transformation, Genetic

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