Isolation of cloned ribosomal protein genes from the yeast Saccharomyces carlsbergensis

Gene
G H BollenR J Planta

Abstract

A colony bank of yeast dna obtained by cloning HindIII-generated fragments of total yeast nuclear DNA in Escherichia coli K-12 with the vector pBR322, was screened with a radioactive RNA probe enriched for a subset of ribosomal protein mRNAs. The selected recombinant DNA molecules were hybridized with poly(A)-containing mRNA under R-loop conditions. From the DNA-RNA hybrids the respective mRNAs were melted off and translated in vitro in a rabbit reticulocyte cell-free system. The translational products were analyzed by immunoprecipitation with antibodies raised against ribosomal proteins. The identity of the ribosomal protein gene products was further established by electrophoresis on two-dimensional gels. At least 15 recombinant DNA molecules were shown to contain ribosomal protein genes. Four of them, i.e. Y65, Y89, Y113 and Y138, have been characterized preliminarily.

References

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Citations

Dec 1, 1984·Current Genetics·P J SchaapR J Planta
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C KruseJ R Warner
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I T ChenD J Roufa
Oct 11, 1982·Nucleic Acids Research·S Fabijanski, M Pellegrini
Nov 25, 1983·Nucleic Acids Research·R J LeerR J Planta
Jul 11, 1984·Nucleic Acids Research·P O O'Connell, M Rosbash
Sep 11, 1984·Nucleic Acids Research·R J LeerR J Planta
Jul 1, 1987·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·V W van HinsberghD C Rijken
Jun 1, 1989·Microbiological Reviews·J R Warner
Dec 1, 1985·Molecular and Cellular Biology·J Y ChenD M Livingston
Dec 1, 1985·Molecular and Cellular Biology·N AbovichM Rosbash
Jul 1, 1983·Journal of Bacteriology·L D Schultz, J D Friesen

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