Isolation of cold-adapted nitrate-reducing fungi that have potential to increase nitrate removal in woodchip bioreactors.

Journal of Applied Microbiology
N Aldossari, S Ishii

Abstract

The aim of this study was to obtain cold-adapted denitrifying fungi that could be used for bioaugmentation in woodchip bioreactors to remove nitrate from agricultural subsurface drainage water. We isolated a total of 91 nitrate-reducing fungal strains belonging to Ascomycota and Mucoromycota from agricultural soil and a woodchip bioreactor under relatively cold conditions (5 and 15°C). When these strains were incubated with 15 N-labelled nitrate, 29 N2 was frequently produced, suggesting the occurrence of co-denitrification (microbially mediated nitrosation). Two strains also produced 30 N2 , indicating their ability to reduce N2 O. Of the 91 nitrate-reducing fungal strains, fungal nitrite reductase gene (nirK) and cytochrome P450 nitric oxide reductase gene (p450nor) were detected by PCR in 34 (37%) and 11 (12%) strains, respectively. Eight strains possessed both nirK and p450nor, further verifying their denitrification capability. In addition, most strains degraded cellulose under denitrification condition. Diverse nitrate-reducing fungi were isolated from soil and a woodchip bioreactor. These fungi reduced nitrate to gaseous N forms at relatively low temperatures. These cold-adapted, cellulose-degrading and nitrate-reducing ...Continue Reading

References

Mar 29, 2001·Archives of Microbiology·Z ZhouH Shoun
Aug 16, 2002·International Microbiology : the Official Journal of the Spanish Society for Microbiology·J PérezJ Martínez
Dec 7, 2005·FEMS Microbiology Ecology·Janna PietikäinenErland Bååth
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Mar 28, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Hirofumi ShounTakayoshi Wakagi
Sep 18, 2012·Chemosphere·Jiří JiroutDana Elhottová
Oct 23, 2012·Applied and Environmental Microbiology·Andrew LongBongkeun Song
Oct 12, 2013·Molecular Ecology·Urmas KõljalgKarl-Henrik Larsson
Aug 5, 2014·Rapid Communications in Mass Spectrometry : RCM·Lena RoheReinhard Well
Feb 2, 2015·Water Research·Ehsan GhaneLarry C Brown
Apr 22, 2015·Scientific Reports·Koki MaedaLaurent Philippot
Mar 13, 2016·Applied and Environmental Microbiology·Steven A HigginsFrank E Löffler
May 22, 2016·Applied and Environmental Microbiology·Amy NovinscakMartin Filion
May 4, 2018·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Feb 7, 2019·Environmental Microbiology·Yanjun MaAngela D Kent
Apr 20, 2019·Frontiers in Microbiology·Jeonghwan JangSatoshi Ishii

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.