Isolation of cypress gibberellin-regulated protein: Analysis of its structural features and IgE binding competition with homologous allergens

Molecular Immunology
Lisa TuppoAdriano Mari

Abstract

The presence in cypress pollen of an important allergen, belonging to the gibberellin-regulated protein (GRP) family, has been suggested for many years. However, it has never been isolated and sometimes the homologous peach allergen, Pru p 7, has been used as a surrogate to perform immunological investigations. The aim of this study has been the isolation and molecular characterization of the GRP contained in the Cupressus sempervirens pollen. This protein, named Cypmaclein, has been purified from the natural source using conventional biochemical methods consisting in different chromatographic separations. Cypmaclein has been identified by direct protein sequencing of the N-terminal region and of internal fragments of the molecule. In SDS-PAGE, its apparent molecular mass is slightly higher than that of Pru p 7. Nevertheless, the mass spectrometry experiments reveal that the exact molecular mass of Cypmaclein (6821.88 Da) is very close to that of Pru p 7 (6909.90 Da). Two regions of Cypmaclein have been sequenced providing 50% of its primary structure. A high overall sequence identity of Cypmaclein with all the analyzed GRP has been observed, although in the N-terminal region the high identity is limited to the homolog of Crypt...Continue Reading

References

Dec 30, 1986·Biochemistry·A Di DonatoG D'Alessio
Nov 6, 2002·The Journal of Allergy and Clinical Immunology·Marija Gavrović-JankulovićRatko M Jankov
Jan 3, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L TuppoM A Ciardiello
Aug 19, 2014·Biopolymers·Lisa TuppoMaria Antonietta Ciardiello
Nov 3, 2015·Journal of Plant Growth Regulation·Peter Hedden, Valerie Sponsel
Mar 16, 2017·Journal of Agricultural and Food Chemistry·Lisa TuppoMaria Antonietta Ciardiello
Apr 13, 2017·Clinical Reviews in Allergy & Immunology·Denis CharpinPascal Poncet
Jul 8, 2017·Immunity, Inflammation and Disease·Naoko InomataMichiko Aihara
Aug 8, 2017·The Journal of Allergy and Clinical Immunology·Hélène SénéchalPascal Poncet
Aug 14, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Naoko InomataMichiko Aihara
Jun 30, 2019·Molecular Immunology·Ivana GiangriecoMaria Antonietta Ciardiello

❮ Previous
Next ❯

Citations

Jun 9, 2020·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Angelica E EhrenbergJonas Lidholm
Jul 22, 2020·Expert Review of Clinical Immunology·Pascal PoncetDenis Charpin
Jun 20, 2020·Clinical and Translational Allergy·Claudia AlessandriAdriano Mari
Aug 14, 2020·Journal of the Science of Food and Agriculture·Marwa YakhlefGabriella Pocsfalvi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.