PMID: 14400127Apr 1, 1960Paper

Isolation of cytoplasmic pituitary granules with gonadotropic activity

The Journal of Biophysical and Biochemical Cytology
M W HartleyH RIS

Abstract

A fraction isolated from the anterior pituitary glands of rats castrate for 8 weeks contained essentially a single cytoplasmic constituent with which the major portion of the gonadotropic hormone activity was associated. The glands were homogenized in an 0.25 M sucrose + 7.3 per cent polyvinylpyrrolidone (PVP) solution and fractionated by differential centrifugation to give a heterogeneous small granule fraction which contained almost all the gonadotropic hormone activity. The active supernatant containing this small granule fraction was separated into layers by isopycnic gradient centrifugation on a continuous 6 to 45 per cent sucrose + 17.5 per cent "diodrast" + 5 x 10(-4)M "versene" gradient at 100,000 g for 2 hours. Three layers were obtained and the pellet from the active bottom layer was sectioned, examined with the electron microscope, and found to contain 200 mmicro granules, mitochondria, ergastoplasm, and other cellular debris. This layer was fractionated further by isopycnic and differential centrifugation to obtain a pellet which contained the major portion of the gonadotropic hormone activity. Because of the heterogeneity of this fraction, due to the contamination of the 200 mmicro granules with mitochondria and ot...Continue Reading

References

Mar 25, 1956·The Journal of Biophysical and Biochemical Cytology·G E PALADE, P SIEKEVITZ
Mar 25, 1957·The Journal of Biophysical and Biochemical Cytology·D H MOORE, P H GRIMLEY
Jan 1, 1958·Proceedings of the Society for Experimental Biology and Medicine·E REID, A SEGALOFF
May 25, 1958·The Journal of Biophysical and Biochemical Cytology·P G SATIR, L D PEACHEY
Jan 25, 1959·The Journal of Biophysical and Biochemical Cytology·P SIEKEVITZ, G E PALADE
Jan 25, 1959·The Journal of Biophysical and Biochemical Cytology·F S LABELLA, J H BROWN
Jul 1, 1949·Proceedings of the Society for Experimental Biology and Medicine·W H McSHAN, R K MEYER

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Citations

Jan 1, 1972·Zeitschrift Für Anatomie Und Entwicklungsgeschichte·T F Gale
Jan 1, 1965·Ergebnisse der Physiologie, biologischen Chemie und experimentellen Pharmakologie·W H McShan, M W Hartley
Jun 1, 1961·The Journal of Biophysical and Biochemical Cytology·D J LUCK
May 1, 1962·The Journal of Cell Biology·A W LINNANEP G NOWLAND
Nov 1, 1962·The Journal of Cell Biology·J F PERDUE, W H McSHAN
Dec 1, 1969·The Journal of Cell Biology·A Costoff, W H McShan
Feb 1, 1961·Transactions of the New York Academy of Sciences·M G FARQUHAR
Jan 1, 1970·Zeitschrift für Zellforschung und mikroskopische Anatomie·A Attramadal

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