Isolation of cytotoxic chondropsins, macrolide lactams from the New-Caledonian marine sponge Psammoclemma sp. and electrospray ion trap multiple stage MS study of these macrolides

Natural Product Research
Camille ChevallierT Sévenet

Abstract

Psammoclemma sp. (Phoriospongiidae) was selected for study through biological screening carried out in New Caledonia. The crude extract of Psammoclemma sp. was highly active on KB cells and its fractionation led to the isolation of two macrolides chondropsin A 1 and 73-deoxychondropsin A 2. The complex structures were elucidated by a combination of spectroscopic methods (NMR, ESI-MS/MS). These known compounds were evaluated for their cytotoxic activity towards several tumor cell lines and exhibited an IC50 of approximately 10(-10) M. Flow-cytometric analysis revealed that chondropsin A 1 and 73-deoxychondropsin A 2 blocked the entry of HL 60 and KB cells into G2/M phase, leading to cell death by apoptosis.

References

Dec 10, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K S Rein, J Borrone

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Citations

Mar 22, 2016·Marine Drugs·Sofia-Eléna MotuhiStéphane Bach
Nov 8, 2007·Bioscience, Biotechnology, and Biochemistry·Daisaku TakemotoShigeki Matsunaga
Feb 15, 2008·Journal of Natural Products·Alberto PlazaCarole A Bewley
Jan 10, 2009·Journal of Natural Products·Ian P HollandIan A van Altena
Apr 15, 2014·Organic Letters·Pierre-Georges EcheverriaPhannarath Phansavath

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Methods Mentioned

BETA
NMR
flow cytometry

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis