PMID: 7544132Feb 1, 1995Paper

Isolation of decidual lymphocytes from chorionic villus samples: phenotypic analysis and growth in vitro

American Journal of Reproductive Immunology : AJRI
M K HaynesJ B Smith

Abstract

Giemsa stained cell isolates prepared from chorionic villus samples (CVS) contain granulated cells morphologically similar to large granular lymphocytes. Phenotypic characterization of these cellular isolates by two-color immunofluorescence and subsequent in vitro culture in the presence of recombinant interleukin-2 (rIL-2) were done in order to determine whether CVS could serve as a source of decidual lymphocytes. A major fraction of the CVS-derived lymphocytes were characterized as decidual NK cells, exhibiting high levels of CD56 expression (CD56+bright), without concomitant expression of CD16. The T cell population present in CVS-derived lymphocytes contained both CD4+ and CD8+ cells in a ratio somewhat reduced compared to that found in peripheral blood. While both T cells and CD56+bright cells from CVS proliferate in vitro in response to rIL-2 alone, preferential growth of CD56+bright cells was accomplished using a selective culture technique wherein co-culture with an irradiated, B lymphoblastoid cell line promoted the growth of CD56+ cells. CVS contains decidual NK cells and T cells that proliferate in response to rIL-2 and/or third party stimulator cells. These culture techniques will allow investigations into the matur...Continue Reading

References

Dec 1, 1991·Immunology Today·A King, Y W Loke
Feb 1, 1990·American Journal of Obstetrics and Gynecology·A King, Y W Loke
Jan 1, 1990·American Journal of Reproductive Immunology : AJRI·J A LataJ B Smith
Feb 1, 1989·American Journal of Reproductive Immunology : AJRI·S DayaD A Clark
Jan 1, 1987·The Journal of Experimental Medicine·K TeshigawaraK A Smith
Nov 23, 1987·Journal of Immunological Methods·A Ritson, J N Bulmer
Jan 1, 1993·American Journal of Reproductive Immunology : AJRI·V P ChernyshovG I Bondarenko
Apr 1, 1993·American Journal of Reproductive Immunology : AJRI·M L VargasE García-Olivares
Jan 1, 1993·Journal of Reproductive Immunology·A King, Y W Loke
Jan 1, 1993·American Journal of Reproductive Immunology : AJRI·T MoriiN Narita
Feb 7, 1953·Nature·N A MITCHISON
Apr 1, 1958·Obstetrics and Gynecology·H HAMPERL, G HELLWEG

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.