Isolation of dehydration-responsive genes in a drought tolerant common bean cultivar and expression of a group 3 late embryogenesis abundant mRNA in tolerant and susceptible bean cultivars

Functional Plant Biology : FPB
Blanca E Barrera-FigueroaBeatriz Xoconostle-Cázares

Abstract

Drought is one of the main constraints for common bean (Phaseolus vulgaris L.) production in Latin America. The aim of this work was to identify upregulated genes in the drought-tolerant common bean cv. Pinto Villa, grown under water-deficit conditions. Twenty-eight cDNAs representing differentially-expressed mRNAs in roots and/or leaves were isolated via suppression subtractive hybridisation. Their expression profiles in plants under intermediate and severe dehydration stress were tested. Three cDNAs corresponded to genes already described as associated to drought stress in P. vulgaris, 12 were known P. vulgaris sequences without previous association with drought response, and 13 were new P. vulgaris sequences. Analysis of the deduced proteins encoded by the cDNAs revealed putative functions in cellular protection, sugar metabolism, and protein synthesis, folding and turnover. Additionally, a new member of group 3 late embryogenesis abundant (LEA) genes (PvLEA3) was cloned and its complete sequence was obtained. Given the lack of reports comparing expression of dehydration-responsive genes in bean cultivars with different response to drought, the expression of PvLEA3 transcript in five bean cultivars from different origin was ...Continue Reading

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Citations

May 1, 2012·Molecular Ecology Resources·Matthew W BlairPrem Sharma
Aug 26, 2009·Plant Signaling & Behavior·Víctor Montero-TaveraBeatriz Xoconostle-Cázares
Oct 27, 2007·The New Phytologist·Lourdes Montalvo-HernándezRoberto Ruíz-Medrano
May 28, 2019·International Journal of Molecular Sciences·Muhammad NadeemLijuan Qiu
Aug 5, 2017·Journal of Agricultural and Food Chemistry·Gabriela Barbosa RossiAna Carolina Maisonnave Arisi

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