Isolation of deoxyribonucleic acid from Mycobacterium avium by rapid nitrogen decompression.

Infection and Immunity
P M Yandell, C McCarthy

Abstract

Deoxyribonucleic acid (DNA) of high molecular weight could be isolated from cells of Mycobacterium avium if the cells were exposed to nitrogen gas at 1,500 lb/in2 for 30 min and then brought to atmospheric pressure by rapid decompression. DNA isolated from the cells had a molecular weight of 4.8 x 10(6) to 17.4 x 10(6). DNA was also released into the fluid in which the cells were suspended during nitrogen decompression. One-half of this DNA, representing 3% of the total DNA phosphorus in the cells had a uniform molecular weight of 4.2 x 10(6). This DNA was linear in conformation, and removal of associated carbohydrates did not change its sedimentation rate. The biological function or significance of the 4-megadalton DNA was not determined.

References

Jan 1, 1977·Advances in Microbial Physiology·W T CoakleyD Lloyd
Dec 29, 1978·Biochemical and Biophysical Research Communications·B B Hemmingsen, E A Hemmingsen
Jun 1, 1979·Infection and Immunity·J T Crawford, J H Bates
Jan 1, 1978·Infection and Immunity·C McCarthy
Apr 2, 1975·Biochimica Et Biophysica Acta·G O HumphreysE S Anderson
Sep 1, 1972·Bacteriological Reviews·R C Clowes
Dec 1, 1972·Journal of Bacteriology·E B HillW M Gross
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D B Clewell, D R Helinski
Nov 1, 1970·Journal of Bacteriology·Y Mizuguchi, T Tokunaga
Mar 4, 1961·Biochimica Et Biophysica Acta·M J HUNTER, S L COMMERFORD
Feb 1, 1962·Journal of Bacteriology·J W FOSTERT A MAAG
Sep 1, 1970·Infection and Immunity·C McCarthy

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Citations

Jun 19, 2007·Biotechnology and Bioengineering·V EspinasseP Gervais
Sep 25, 2008·Biotechnology and Bioengineering·V EspinasseP Gervais

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