Isolation of detergent-extracted Sendai virus proteins by gel-filtration, ion-exchange and reversed-phase high-performance liquid chromatography and the effect on immunological activity

Journal of Chromatography
G W WellingS Welling-Wester

Abstract

Virus envelope proteins were isolated from Triton X-100 extracts of purified Sendai virions by gel-filtration, ion-exchange and reversed-phase high-performance liquid chromatography (HPLC). The fusion protein F, the matrix protein M and the tetrameric and dimeric form of the HN protein were isolated by gel-filtration HPLC with a solvent containing 0.1% sodium dodecyl sulphate. HN and F were also isolated by ion-exchange HPLC with 0.1% Triton X-100 in the eluent. Reversed-phase HPLC was performed on a C1 column with acetonitrile as the organic solvent. Especially the F1 and F2 component of the fusion protein F were obtained in pure form. The immunological activity of the proteins after HPLC was determined with an enzyme-linked immunosorbent assay (ELISA). After gel-filtration and ion-exchange HPLC, proteins still reacted with antiserum to the intact virus while proteins purified by reversed-phase HPLC did not react.

References

Mar 25, 1975·Biochimica Et Biophysica Acta·A Helenius, K Simons
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Jan 1, 1983·Methods in Enzymology·F E Regnier

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Citations

Sep 1, 1994·Journal of Biochemical and Biophysical Methods·S KhamlichiO Bertrand
Feb 1, 1982·Journal of Reproductive Immunology·K M Ebert, D L Black
Dec 26, 1986·Journal of Chromatography·M KunitaniL R Snyder
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·K RamaniD P Sarkar
Jan 1, 1995·AIDS Research and Human Retroviruses·P J BugelskiT K Hart
Jun 26, 1987·Journal of Chromatography·G W WellingS Welling-Wester
Apr 3, 1987·Journal of Chromatography·Y KatoT Hashimoto
Jul 17, 1987·Journal of Chromatography·G W WellingS Welling-Wester

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