Isolation of early immunoglobulin lambda-like gene transcripts in human fetal liver

European Journal of Immunology
C SchiffM Fougereau

Abstract

In an attempt to identify early events of human Ig gene expression, we have screened a human fetal liver cDNA library (less than 90 days of gestation) with C mu-, C gamma-, C kappa-, C lambda-specific probes and we report the characterization of two clones, F lambda 1 and F lambda 8, that hybridized with a human C lambda gene. These two clones, which are only 85% homologous to the functional C lambda genes, were shown to be additional nonallelic members of the 14.1/16.1 C lambda-like family. Using pulsed field gel electrophoresis these three C lambda-like genes were shown to be present on a 200-kb DNA fragment, defining a cluster distinct from that of the C lambda one. F lambda 1 and F lambda 8 contained an identical C lambda-like region, and differed from each other by a splicing event which joins a J lambda-like to the C lambda-like exon in the F lambda 1 clone in the absence of any rearrangement. Homologies observed between F lambda 1 and the mouse lambda 5 gene suggest that this human clone may contain the exon 2 and 3 equivalents of lambda 5. Since lambda 5 is selectively expressed in pre-B cells, our proposal is also supported by the early expression of this clone, together with the presence of full-length mu and gamma tr...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1986·The Journal of Experimental Medicine·U StorbR L Brinster
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H KubagawaM D Cooper
Jan 1, 1988·Annales De L'Institut Pasteur. Immunology·C TonnelleM Fougereau
Feb 1, 1986·The Journal of Experimental Medicine·H ChangG F Hollis
Feb 1, 1986·Immunological Reviews·F W AltG D Yancopoulos
Nov 6, 1987·Science·H W SchroederR M Perlmutter
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P DariavachM P Lefranc
Oct 12, 1987·Nucleic Acids Research·C D Reymond
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein

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Citations

Aug 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·C SchiffM Fougereau
Jul 1, 1991·Molecular Immunology·M MililiC Schiff
Feb 1, 1993·Immunology Today·F MelchersA Rolink
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·N NishimotoM D Cooper
Oct 1, 1990·The Journal of Experimental Medicine·T OhnoM D Cooper
Jan 1, 1992·International Reviews of Immunology·C SchiffM Fougereau
Jan 1, 1992·International Reviews of Immunology·H H Wortis
May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M E DonohoeB B Blomberg

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