PMID: 9661050Jul 14, 1998Paper

Isolation of EpH4 mammary epithelial cell subpopulations which differ in their morphogenetic properties

In Vitro Cellular & Developmental Biology. Animal
R MontesanoL Orci

Abstract

EpH4 is a nontumorigenic cell line derived from spontaneously immortalized mouse mammary gland epithelial cells (Fialka et al., 1996). When grown in collagen gels, EpH4 cells give rise to different types of structures, e.g., solid cords or branching tubes. By removing and subsequently dissociating single three-dimensional colonies of defined morphology, we have isolated six clonal subpopulations of EpH4 cells which display distinct morphogenetic properties in collagen gel cultures. Thus, cells from the H1B clone form branching cords devoid of a central lumen, K3A3 cells form cords enclosing small multifocal lumina, and J3B1 cells form large cavitary structures containing a wide lumen. I3G2 cells form either cords or tubes, depending on the type of serum added to the culture medium. Finally, when grown in serum-free medium, Bela cells form spherical cysts, whereas Be4a cells form long, extensively branched tubes. In additional assays of morphogenesis, i.e., cell sandwiching between two collagen gels or culture on a thick layer of Matrigel (a laminin-rich extracellular matrix), all clones form epithelial-cell lined cavitary structures, except H1B cells which are unable to generate lumina under these conditions. The EpH4 sublines ...Continue Reading

References

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Citations

Mar 17, 2006·Breast Cancer Research and Treatment·Geneviève Vaudan VutskitsAndré-Pascal Sappino
Dec 27, 2006·Biochemical and Biophysical Research Communications·Roberto Montesano
Aug 10, 2012·Molecular Biology of the Cell·Maryline AllegraGeorge Mavrothalassitis
Jun 11, 2004·Médecine sciences : M/S·Ahmed Zahraoui
Oct 20, 1999·Developmental Biology·T C KlinowskaC H Streuli
Mar 13, 2014·Seminars in Cell & Developmental Biology·Mirjam M Zegers
Jul 16, 2008·Biochemical and Biophysical Research Communications·Roberto MontesanoHerbert Schramek
Nov 4, 2000·International Journal of Cancer. Journal International Du Cancer·R BachelierA Vincent
Oct 26, 2016·Scientific Reports·Stuart A CainCay M Kielty
Oct 3, 2002·Oncogene·Clémence MagnardNicole Dalla Venezia
Aug 29, 2014·American Journal of Physiology. Cell Physiology·Priscilla SouliéEric Féraille
Dec 5, 2008·American Journal of Physiology. Renal Physiology·Roberto MontesanoEric Féraille
Nov 5, 2002·Journal of Cell Science·Roberto Montesano, Priscilla Soulié

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