Isolation of Escherichia coli O157 from raw meat products

Letters in Applied Microbiology
F J BoltonJ K Williamson

Abstract

This study has evaluated an enrichment and four subculture procedures for detection of Escherichia coli O157 in raw meat products. The combination of enrichment in modified tryptone broth incubated at 42 degrees C for 6 h, followed by immunomagnetic separation and subculture on to cefixime, tellurite sorbitol MacConkey agar was the most sensitive and selective procedure. Traditional subculture using 10 microliters and 100 microliters inocula and culture of centrifuged deposits were less satisfactory. A most probable number method was used to enumerate E. coli O157 in naturally contaminated samples associated with human cases. The results indicated that the samples contained < 0.3 to 2300 cfu g-1 of E. coli O157 which confirms that the infective dose for this organism is low.

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Citations

Oct 24, 2006·Environmental Monitoring and Assessment·P W Ramteke, Suman Tewari
Apr 27, 2001·International Journal of Food Microbiology·H H WangR Kuang
Aug 6, 2002·International Journal of Food Microbiology·Elodie VoitouxBertrand Lombard
Sep 14, 2002·International Journal of Food Microbiology·C Bell
May 15, 2003·International Journal of Food Microbiology·Susanna TyöppönenTiina Mattila-Sandholm
Jan 29, 1999·International Journal of Food Microbiology·E de Boer
Sep 6, 2000·European Journal of Biochemistry·H Beinert
May 25, 1999·Microbiology and Immunology·S SataS Yamai
Jul 6, 2000·Symposium Series· De Boer E, A E Heuvelink
Apr 5, 2011·Meat Science·Rubén GordilloMar Rodríguez
Jun 24, 2014·Saudi Journal of Biological Sciences·Mohamed Fawzy Ramadan HassanienKahled M El-Zahar
Aug 5, 1999·Letters in Applied Microbiology·L RestainoD R Allison
Dec 12, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Richard D OberstJan M Sargeant
May 4, 2004·The Veterinary Record·N CollinsJ Schumacher
Sep 26, 2009·Journal of Applied Microbiology·B FremauxC Rozand
Feb 8, 2006·Die Naturwissenschaften·Patrizia D'EttorreFrancis L W Ratnieks
Jun 13, 2003·Journal of Food Protection·Min-Suk RheeDong-Hyun Kang
Mar 20, 2002·Journal of Food Protection·Marian R Wachtel, Amy O Charkowski
Oct 29, 2017·Environmental Science and Pollution Research International·Mohamed E Abd El-HackIslam M Saadeldin
Jul 16, 2010·Food Microbiology·Vicky JassonMieke Uyttendaele

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