Isolation of estrogen-responsive genes with a CpG island library.

Molecular and Cellular Biology
T WatanabeM Muramatsu

Abstract

In order to isolate novel estrogen-responsive genes, we utilized a CpG island library in which the regulatory regions of genes are enriched. CpG islands were screened for the ability to bind to a recombinant estrogen receptor protein with a genomic binding site (GBS) cloning method. Six CpG islands were selected, and they contained perfect, imperfect, and/or multiple half-palindromic estrogen-responsive elements (EREs). Northern blot analysis of various human cells showed that all these genomic fragments hybridized to specific mRNAs, suggesting that the genes associated with these EREs might be transcribed in human cells. Then cDNAs associated with two of them, EB1 and EB9, were isolated from libraries of human placenta and MCF-7 cells derived from a human breast cancer, respectively. Both transcripts were increased by estrogen in MCF-7 cells. The increase is inhibited by actinomycin D but not by cycloheximide, indicating that no protein synthesis is required for the up-regulation. The cDNA associated with EB1 encodes a 114-amino-acid protein similar to the cytochrome c oxidase subunit VIIa, named COX7RP (cytochrome c oxidase subunit VII-related protein). The cDNA associated with EB9 is homologous only to an express sequence ta...Continue Reading

References

Apr 15, 1977·Science·W E StumpfG Aumüller
May 1, 1992·Journal of Neurochemistry·E Bettini, A Maggi
Aug 1, 1992·Genomics·F LarsenH Prydz
Feb 11, 1992·Nucleic Acids Research·M R Ponce, J L Micol
Aug 11, 1991·Nucleic Acids Research·S InoueM Muramatsu
Nov 22, 1990·Nature·A P GouldR A White
Nov 15, 1990·International Journal of Cancer. Journal International Du Cancer·M PoutanenR Vihko
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J P HendrickL E Rosenberg
Nov 1, 1988·Trends in Genetics : TIG·S Green, P Chambon
Jan 1, 1988·Annals of the New York Academy of Sciences·R O PoytonL E Farrell
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M BerryP Chambon
Mar 25, 1987·Nucleic Acids Research·S KoikeM Muramatsu
Jul 20, 1987·Journal of Molecular Biology·M Gardiner-Garden, M Frommer
Nov 1, 1973·Journal of the National Cancer Institute·H D SouleM Brennan
Sep 15, 1973·The Journal of Comparative Neurology·D Pfaff, M Keiner
Feb 1, 1971·Cancer·R M McAllisterB H Landing
Feb 11, 1983·Nucleic Acids Research·D N CooperA P Bird
Jun 15, 1982·European Journal of Biochemistry·P Merle, B Kadenbach
Jan 15, 1983·International Journal of Cancer. Journal International Du Cancer·N MorinagaT Kuwata
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F Antequera, A Bird
Nov 1, 1995·American Journal of Respiratory Cell and Molecular Biology·E ClaudioP S Lazo
Sep 15, 1993·Biochemical and Biophysical Research Communications·A OrimoH Orimo
Sep 1, 1994·Molecular and Cellular Endocrinology·Y LiuR Zarnegar
Mar 1, 1994·Nature Genetics·S H CrossA P Bird
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S InoueM Muramatsu
Apr 5, 1996·The Journal of Biological Chemistry·D E Phelps, G R Dressler
May 24, 1996·The Journal of Biological Chemistry·F SegadeP S Lazo
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·R E DavisW D Parker

❮ Previous
Next ❯

Citations

Jul 10, 2008·Molecular and Cellular Endocrinology·Pallob KunduMansoureh Eghbali
Jan 22, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Wei Dong HanHai Jing Song
Oct 24, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Patrick AugereauVincent Cavaillès
May 22, 2009·Journal of Neurochemistry·Susanne Arnold, Cordian Beyer
Feb 12, 2009·International Journal of Urology : Official Journal of the Japanese Urological Association·Tetsuya FujimuraSatoshi Inoue
Nov 26, 2008·International Journal of Cancer. Journal International Du Cancer·Jinpei KumagaiSatoshi Inoue
May 3, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Véronique St-LaurentAndré Tremblay
Jun 11, 2002·Biochemical and Biophysical Research Communications·Joel RousseauBenoit Guérette
Dec 4, 2014·Cell Metabolism·Arnaud MourierDusanka Milenkovic
May 13, 2008·Mechanisms of Development·Jun-ichi KyunoElizabeth A Jones
Jan 18, 2005·Journal of Carcinogenesis·Quentin Felty, Deodutta Roy
Jul 12, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T AokiM Makuuchi
Sep 15, 2010·Molecular and Cellular Endocrinology·Hui Gao, Karin Dahlman-Wright
Aug 24, 2016·Scientific Reports·Kezhong ZhangLawrence I Grossman
Mar 23, 2005·Archives of Histology and Cytology·Kazuhiro Ikeda, Satoshi Inoue
May 1, 2007·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·X-H ZhouY-J Wu
Jul 9, 2004·The Journal of Veterinary Medical Science·Yasuhiko OkamuraYasuho Taura
Mar 14, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Rocio SanchezSylvie Mader
Jul 8, 2003·International Journal of Cancer. Journal International Du Cancer·Satoru TakahashiSatoshi Inoue
Feb 3, 2007·Molecular Nutrition & Food Research·Brian G Chrzan, Peter G Bradford
Jul 22, 2009·The Journal of Clinical Investigation·Constantin RüderArmin Rehm
Jul 29, 2005·Journal of Gastroenterology and Hepatology·Munechika EnjojiMakoto Nakamuta
Mar 17, 2001·Nature Reviews. Genetics·L A Pennacchio, E M Rubin
Apr 25, 2006·Nature Reviews. Cancer·Yongfeng Shang
Jan 7, 2006·Molecular Endocrinology·Jason S Carroll, Myles Brown
Mar 2, 2012·International Journal of Urology : Official Journal of the Japanese Urological Association·Hidenori Kawashima, Tatsuya Nakatani

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.