Isolation of Exosome-Like Nanoparticles and Analysis of MicroRNAs Derived from Coconut Water Based on Small RNA High-Throughput Sequencing

Journal of Agricultural and Food Chemistry
Zhehao ZhaoPing Li

Abstract

In this study, the presence of microRNAs in coconut water was identified by real-time polymerase chain reaction (PCR) based on the results of high-throughput small RNA sequencing. In addition, the differences in microRNA content between immature and mature coconut water were compared. A total of 47 known microRNAs belonging to 25 families and 14 new microRNAs were identified in coconut endosperm. Through analysis using a target gene prediction software, potential microRNA target genes were identified in the human genome. Real-time PCR showed that the level of most microRNAs was higher in mature coconut water than in immature coconut water. Then, exosome-like nanoparticles were isolated from coconut water. After ultracentrifugation, some particle structures were seen in coconut water samples using 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate fluorescence staining. Subsequent scanning electron microscopy observation and dynamic light scattering analysis also revealed some exosome-like nanoparticles in coconut water, and the mean diameters of the particles detected by the two methods were 13.16 and 59.72 nm, respectively. In conclusion, there are extracellular microRNAs in coconut water, and their levels are ...Continue Reading

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Feb 7, 2019·Food & Function·Sophie Rome
May 24, 2019·Advances in Nutrition·Lin ZhangYong-Liang Zhang
Dec 5, 2019·Advances in Nutrition·Javaria MunirSeongho Ryu
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Oct 22, 2020·Seminars in Cancer Biology·Alberto DávalosDavid de Gonzalo-Calvo
May 1, 2019·Molecular Pharmaceutics·Xingyi ChenJiujiu Yu

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