Isolation of expressed sequence tags of Agaricus bisporus and their assignment to chromosomes.

Applied and Environmental Microbiology
A S SonnenbergL J Van Griensven

Abstract

The genome of the cultivated basidiomycete Agaricus bisporus Horst U1 and of its homokaryotic parents has been characterized by using an optimized method of pulsed-field gel electrophoresis. Expressed sequence tags obtained as expressed cDNAs from a primordial tissue-derived cDNA library and a number of previously isolated genes were used to identify the individual chromosomes of the parental lines of Horst U1. The genome consists of 13 chromosomes, and its total size is 31 Mb. For those chromosomes that could not be resolved by contour-clamped homogeneous electric field electrophoresis, the segregation of marker genes was studied in a set of 86 homokaryotic offspring of Horst U1. At least two markers were assigned to each individual chromosome. In this way all individual chromosomes were unequivocally identified. The large size difference observed between the homologous chromosomes IX, harboring the rDNA repeat, was shown to be largely due to a higher copy number of rDNA in parental strain H97 than in parental strain H39.

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Citations

Dec 1, 2000·Applied and Environmental Microbiology·L M LarrayaL Ramírez
Jul 31, 2002·Applied and Environmental Microbiology·María M PeñasAntonio G Pisabarro
Aug 30, 2002·Applied and Environmental Microbiology·Isabelle LacourtFrancis Martin
Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Emmanuelle MorinFrancis Martin
Jul 31, 1999·Applied and Environmental Microbiology·A S SonnenbergL J Van Griensven
Jul 31, 1999·Applied and Environmental Microbiology·L M LarrayaL Ramírez
Mar 15, 2006·Fungal Genetics and Biology : FG & B·Sang-Kyu ParkAntonio G Pisabarro

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