PMID: 8587863Oct 1, 1995Paper

Isolation of fetal cells from transcervical samples by micromanipulation: molecular confirmation of their fetal origin and diagnosis of fetal aneuploidy

Prenatal Diagnosis
B TutschekM Adinolfi

Abstract

Transcervical cell (TCC) samples have been shown to contain fetal cells amenable to molecular analysis. However, the presence of 'contaminating' maternal cells limits their use for prenatal diagnoses. In this report we show that clumps of fetal cells can be isolated from transcervical samples by micromanipulation and tested by fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR). Out of 129 clumps, isolated from mucus aspirates and transcervical lavages from 29 patients, 29 clumps from 11 patients were found to be exclusively of fetal origin as judged by the detection of chromosome 21-specific polymorphic DNA markers and Y-derived DNA sequences by PCR and FISH. One case of a male triploid fetus, diagnosed by the analysis of TCC samples obtained by mucus aspiration and lavage, was confirmed by testing clumps of cells isolated by micromanipulation.

References

May 1, 1975·American Journal of Obstetrics and Gynecology·S A RhineJ F Thompson
Feb 1, 1991·Genomics·Y NakahoriY Nakagome
Sep 7, 1994·Annals of the New York Academy of Sciences·D W Bianchi
Aug 1, 1995·Obstetrics and Gynecology·J KingdomM Adinolfi
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Citations

Feb 8, 2000·Prenatal Diagnosis·J C Harper, D Wells
Jan 26, 2002·Prenatal Diagnosis·Riccardo CioniGianfranco Scarselli
Apr 3, 2007·Molecular Diagnosis & Therapy·Cecilia BussaniGianfranco Scarselli
Feb 11, 2000·Clinical Genetics·V CiriglianoM Adinolfi
Dec 12, 2001·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·R C JordanJ A Regezi
Feb 1, 1997·Obstetrical & Gynecological Survey·D M ShererM Y Divon
Apr 19, 2008·Obstetrical & Gynecological Survey·F M NdumbeJ C Konje
Feb 14, 1998·Journal of Clinical Pathology·J J O'LearyM A Dada
Apr 1, 1997·Journal of Medical Genetics·J SherlockM Adinolfi
Sep 15, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Debbie Mantzaris, David S Cram
May 18, 2010·Early Human Development·Stavros SifakisAliza Amiel
Apr 22, 2005·BJOG : an International Journal of Obstetrics and Gynaecology·Mandy G Katz-JaffeDavid S Cram
Jun 1, 1997·Prenatal Diagnosis·M AdinolfiC Rodeck
Feb 8, 2003·Prenatal Diagnosis·Riccardo CioniGianfranco Scarselli
Mar 1, 1997·Prenatal Diagnosis·Y P DaryaniM A Patton
Feb 26, 2005·American Journal of Medical Genetics. Part a·Elissa C OsborneD S Cram
Jan 13, 2006·The Australian & New Zealand Journal of Obstetrics & Gynaecology·Debbie MantzarisGab Kovacs
Apr 8, 2006·Current Opinion in Obstetrics & Gynecology·Farideh Z Bischoff, Joe Leigh Simpson

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