Isolation of freeze-tolerant laboratory strains of Saccharomyces cerevisiae from proline-analogue-resistant mutants

Applied Microbiology and Biotechnology
H TakagiShigeru Nakamori

Abstract

Since some amino acids, polyols and sugars in cells are thought to be osmoprotectants, we expected that several amino acids might also contribute to enhancing freeze tolerance in yeast cells. In fact, proline and charged amino acids such as glutamate, arginine and lysine showed a marked cryoprotective activity nearly equivalent to that of glycerol or trehalose, both known as major cryoprotectants for Saccharomyces cerevisiae. To investigate the cryoprotective effect of proline on the freezing stress of yeast, we isolated proline-analogue-resistant mutants derived from a proline-non-utilizing strain of S. cerevisiae. When cultured in liquid minimal medium, many mutants showed a prominent increase, two- to approximately tenfold, in cell viability compared to the parent after freezing in the medium at -20 degrees C for 1 week. Some of the freeze-tolerant mutants were found to accumulate a higher amount of proline, as well as of glutamate and arginine which are involved in proline metabolism. It was also observed that proline-non-utilizer and the freeze-tolerant mutants were able to grow against osmotic stress. These results suggest that the increased flux in the metabolic pathway of specific amino acids such as proline is effectiv...Continue Reading

Citations

May 17, 2007·Applied Microbiology and Biotechnology·Shingo IzawaYoshiharu Inoue
Jun 20, 2013·Applied Microbiology and Biotechnology·Thi Mai Hoa Bach, Hiroshi Takagi
Nov 11, 2009·Journal of Industrial Microbiology & Biotechnology·Dragana StanleyGrant A Stanley
Mar 23, 2005·FEMS Yeast Research·Z Petek CakarUwe Sauer
Jun 4, 1999·Trends in Biotechnology·F Randez-GilJ A Prieto
Sep 7, 2001·Trends in Plant Science·F A HoekstraJ Buitink
Oct 20, 2005·Journal of Bioscience and Bioengineering·Yuko MoritaHiroshi Takagi
Jul 22, 2008·Applied and Environmental Microbiology·Tomohiro KainoHiroshi Takagi
Dec 8, 2005·Applied and Environmental Microbiology·Hiroshi TakagiYoshito Kubo
Mar 28, 2012·Bioscience, Biotechnology, and Biochemistry·Tomohiro KainoHiroshi Takagi
May 2, 2001·Bioscience, Biotechnology, and Biochemistry·R HirasawaH Kawai
Feb 1, 2014·Applied Biochemistry and Biotechnology·Xiaojian ShiJining Wang
Apr 17, 2014·Journal of Agricultural and Food Chemistry·Tao BoShi-Ru Jia
Sep 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Chikara HoshikawaHiroshi Takagi
Aug 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Michiyo Nomura, Hiroshi Takagi
Mar 8, 2013·Annual Review of Food Science and Technology·Francisca Randez-GilJosé A Prieto
Nov 2, 2011·International Journal of Food Microbiology·Yu SasanoHiroshi Takagi
Mar 12, 2016·Journal of Industrial Microbiology & Biotechnology·Jian DongDongguang Xiao
Oct 12, 2007·Journal of Applied Microbiology·M F KronbergM A Galvagno
Oct 19, 2006·Journal of Bioscience and Bioengineering·Kumio YokoigawaKenji Soda

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