PMID: 6113007Apr 22, 1981Paper

Isolation of frog heart sarcolemma possessing (Ca2+ + Mg2+)-ATPase and Ca2+ pump activities

Biochimica Et Biophysica Acta
N C Morcos

Abstract

The presence of a (Ca2+ + Mg2+)-ATPase and Ca2+ pump in frog heart sarcolemma was investigated. Frog cardiac sarcolemma was isolated by using a modification of a recent procedure for isolating sarcolemma from dog heart (Morcos, N.C. and Drummond, G.I. (1980) Biochim. Biophys. Acta 598, 17-39). Despite the sparseness of sarcoplasmic reticulum in frog heart, this tissue possessed (Ca2+ + Mg2+)-ATPase activity which appeared endogenous to the sarcolemma. The purified sarcolemmal vesicles also possessed ouabain-sensitive (Na+ + K+)-ATPase, Na+ -Ca2+ exchange activity and Ca2+ pump activity. Inclusion of ionophore A23187 into the assay medium resulted in an approximately 5-fold increase in (Ca2+ + Mg2+)-ATPase which may be due to removal of inhibitory concentrations of Ca2+ accumulated within the membrane vesicles by an ATP-dependent process. Vesicles pre-loaded with 45Ca2+, via Na+-Ca2+ exchange, lost 92% of their Ca2+ when ATP was present and only 60% in the absence of ATP. The results indicate that frog heart sarcolemma possesses a (Ca2+ + Mg2+)-ATPase and an ATP-dependent Ca2+ pump which may be involved in the outward pumping of Ca2+ from the heart cell.

References

Nov 1, 1976·Canadian Journal of Biochemistry·P J St Louis, P V Sulakhe
Apr 21, 1975·Biochimica Et Biophysica Acta·G Meissner
Jun 1, 1976·Journal of Biochemistry·N S DhallaJ A Harrow
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R L BarchiM Wong
Jan 1, 1978·Canadian Journal of Biochemistry·N C Morcos, A L Jacobson
May 1, 1979·Canadian Journal of Physiology and Pharmacology·N C Morcos, A L Jacobson
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J P Reeves, J L Sutko
Mar 1, 1971·Journal of Ultrastructure Research·M S Forbes, N Sperelakis
May 1, 1967·The American Journal of Anatomy·L V Leak
Nov 1, 1971·The American Journal of Physiology·K I ShineG A Langer
Jan 1, 1969·Annual Review of Physiology·E H Sonnenblick, A C Stam
Jul 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·E PageB Power
Sep 1, 1970·The Journal of Cell Biology·K M Baldwin
Dec 1, 1968·The Journal of General Physiology·L E Bailey, P E Dresel
May 8, 1980·Biochimica Et Biophysica Acta·N C Morcos, G I Drummond
Feb 21, 1980·Nature·P Caroni, E Carafoli

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Citations

Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·R Volkmann
Jan 1, 1983·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·H Gesser, E Jørgensen
Jan 1, 1986·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·R VolkmannL E Ericson

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