PMID: 7026975Jan 1, 1981Paper

Isolation of fusions between the lac genes and several genes of the exu regulon: analysis of their regulation, determination of the transcription direction of the uxaC-uxaA operon, in Escherichia coli K-12

Molecular & General Genetics : MGG
N Hugouvieux-Cotte-Pattat, J Robert-Baudouy

Abstract

Gene fusions between the lac structural genes and different genes of the hexuronate system were isolated by the two methods described by Casadaban (1976, 1979). Mud (Apr lac) mutants which have the lac genes fused to the regulatory region of exuT, uxaC, uxaA and uxaB genes were constructed. Separately, the lac genes carried by a lambda plac-Mu hybrid phage were placed into a Mucts prophage inserted into uxaC gene and fusions were obtained, by selection of deletions putting the lac genes under the control of the uxaC regulatory elements. Induction of the lysogen led to the isolation of a lambda transducing phage bearing this uxaC-lac fusion. In all the fusion strains, beta-galactosidase expression was shown to be inducible by the natural inducers of hexuronate system enzymes, sensitive to catabolite repression and under the negative control of the exuR regulatory gene. The mutants with Mu insertion were used to establish the direction of transcription of the uxaC-uxaA operon, which is counter-clockwise on the circular genetic map of Escherichia coli (from uxaC to uxaA).

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M J Casadaban, S N Cohen
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A UllmannA Danchin
Jan 1, 1976·Annual Review of Genetics·A I Bukhari
Jan 1, 1975·Biochimie·J M Robert-BaudouyF R Stoeber
Nov 14, 1975·Science·M M Howe, E G Bade
Mar 15, 1974·European Journal of Biochemistry·J M Robert-BaudouyF R Stoeber
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Sep 1, 1980·Journal of Bacteriology·R PortalierF Stoeber

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Citations

Jan 1, 1983·Molecular & General Genetics : MGG·N Hugouvieux-Cotte-Pattat, J Robert-Baudouy
Jul 11, 1998·Journal of Bacteriology·N Peekhaus, T Conway
Dec 1, 2004·EcoSal Plus·M-A Mandrand-BerthelotN Hugouvieux-Cotte-Pattat
Jun 1, 1983·Microbiological Reviews·B J Bachmann
May 1, 1983·Journal of Bacteriology·N Hugouvieux-Cotte-PattatJ Robert-Baudouy
Jun 1, 1983·Journal of Bacteriology·F Van Gijsegem, A Toussaint
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

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