Isolation of glycopeptides with skin test activity from dermatophytes.

Infection and Immunity
S A Moser, J D Pollack

Abstract

By using ethylene glycol extraction of whole submerged cultures followed by Sephadex G-200 and diethylaminoethyl-Sephadex chromatography, we isolated four distinct glycopeptides from Trichophyton mentagrophytes, T. rubrum, and Microsporum canis. Chemical analyses revealed that these glycopeptides contained mostly carbohydrate (42.5 to 81.6%) and protein (4.3 to 11.3%), with lesser amounts of phosphorus (0.4 to 6.0%) and hexosamines (0.3 to 0.6%). Based upon total carbohydrate and monosaccharide content, these dermatophyte glycopeptides could be divided into two chemical groups: glucopeptides (DSI1) and mannopeptides (DSI2, DSII1, and DSII2). The mannopeptides and glucopeptides of each species of dermatophyte were not significantly different chemically from those derived from the other two dermatophyte species studied. Skin testing of DSI1-glycopeptides or DSI2-mannopeptides in immunized guinea pigs indicated that only the DSI2-mannopeptides elicited a delayed hypersensitivity reaction. Skin testing T. mentagrophytes 62-infected guinea pigs with the four purified DS-glycopeptides, as well as earlier fractions from the purification scheme, derived from T. mentagrophytes, T. rubrum, and M. canis, again indicated that only the DSI2...Continue Reading

References

Aug 1, 1976·Infection and Immunity·M T ArnoldF Blank
Jun 1, 1974·Bacteriological Reviews·S F GrappelF Blank
Apr 1, 1968·Journal of Bacteriology·S F GrappelC T Bishop
Jan 1, 1971·The Journal of Investigative Dermatology·R J YuF Blank
Jan 1, 1968·Dermatologica·H L SafersteinC T Bishop
May 1, 1956·Journal of Neurochemistry·L SVENNERHOLM
Jul 16, 1963·Biochimica Et Biophysica Acta·S A BARKERJ H HOLDEN
Jan 1, 1933·The Biochemical Journal·L A Elson, W T Morgan

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Citations

Dec 1, 1985·The Australasian Journal of Dermatology·M V Dahl
Jan 1, 1989·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·K Kostro
Jan 18, 2005·Clinical Microbiology Reviews·Judith A Woodfolk
Jul 1, 1995·Clinical Microbiology Reviews·D K Wagner, P G Sohnle

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