Isolation of haemorphin-related peptides from filter membranes collected in connection with haemofiltration of human subjects

Journal of Chromatography. a
G NybergF Nyberg

Abstract

This paper describes the extraction and isolation from dialysis filters of two peptides containing the opioid active sequence haemorphin-7. The filter devices were obtained from uraemic patients subjected to haemofiltration. Following acidic extraction of the filter membranes the peptides were purified by size-exclusion, ion-exchange chromatography and finally by reversed-phase chromatography using different columns and different chromatographic systems. The purification was guided by radioimmunoassay and the structure of the final products was elucidated by N-terminal sequencing and fast-atom bombardment mass spectrometry as well as micro-electrospray mass spectrometry. The isolated peptides were suggested to be identical to fragments 1-41 and 32-41 of the beta-chain of human haemoglobin.

References

Jan 1, 1992·International Urology and Nephrology·A A AlarabiB Wikström
Apr 30, 1992·Biochemical and Biophysical Research Communications·E L GlämstaF Nyberg
May 1, 1982·Acta Psychiatrica Scandinavica·H A Jørgensen, C Cappelen

Citations

Aug 15, 1998·Mass Spectrometry Reviews·T Niwa
Oct 14, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Batia KaplanBrian M Martin
Mar 10, 2001·Biochemical and Biophysical Research Communications·J SzikraA Borsodi
Jul 8, 1998·FEBS Letters·O N YatskinV T Ivanov
Aug 22, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anthony L AlbistonSiew Yeen Chai
Nov 13, 2020·Frontiers in Pharmacology·Mathias Hallberg, Mats Larhed

Related Concepts

Hemorphin 7
Chromatography
High Pressure Liquid Chromatography Procedure
Globin
Venovenous Hemofiltration
Eryhem
Peptide Fragments
Uremia
Spectrometry, Mass, Fast Atom Bombardment
Homologous Sequences

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