Isolation of haemorphin-related peptides from filter membranes collected in connection with haemofiltration of human subjects

Journal of Chromatography. a
G NybergF Nyberg


This paper describes the extraction and isolation from dialysis filters of two peptides containing the opioid active sequence haemorphin-7. The filter devices were obtained from uraemic patients subjected to haemofiltration. Following acidic extraction of the filter membranes the peptides were purified by size-exclusion, ion-exchange chromatography and finally by reversed-phase chromatography using different columns and different chromatographic systems. The purification was guided by radioimmunoassay and the structure of the final products was elucidated by N-terminal sequencing and fast-atom bombardment mass spectrometry as well as micro-electrospray mass spectrometry. The isolated peptides were suggested to be identical to fragments 1-41 and 32-41 of the beta-chain of human haemoglobin.


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Related Concepts

Hemorphin 7
High Pressure Liquid Chromatography Procedure
Venovenous Hemofiltration
Peptide Fragments
Spectrometry, Mass, Fast Atom Bombardment
Homologous Sequences

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