Isolation of hepatocytes from livers from non-heart-beating donors for cell transplantation

Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
Robin D HughesPaolo Muiesan

Abstract

One of the limitations to hepatocyte transplantation is the restricted availability of donor liver tissue. The aim of this study was to evaluate livers from non-heart-beating donors (NHBDs) as a source of hepatocytes for cell transplantation. A total of 20 livers/segments obtained from NHBD were perfused under good manufacturing practices using a standard collagenase digestion method. The donor liver median warm ischemia time was 15 minutes (range, 11-40 minutes), and cold ischemia time was 13 hours (range, 6-30 hours) prior to cell isolation. The cell viability of the hepatocytes obtained was 52% (1-81%), with a yield of 2.2 x 10(6)(0.2-29.7 x 10(6)) cells per gram of tissue. There was a significant negative correlation between hepatocyte viability and length of both warm ischemia (r = -0.544, P = 0.013) and cold ischemia (r = -0.510, P = 0.022). Preliminary experiments were performed on the viability testing of NHBD livers based on digestion of needle biopsies with collagenase and assessment of the hepatocytes produced. Two of the NHBD cell preparations, which had been cryopreserved, were used as part of a series of cell infusions for hepatocyte transplantation. A 3.5-yr-old girl with Crigler-Najjar syndrome type I received 9...Continue Reading

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Citations

Apr 7, 2010·Nature Reviews. Gastroenterology & Hepatology·Anil DhawanRagai R Mitry
Nov 16, 2011·Transplantation·Robin D HughesAnil Dhawan
Oct 30, 2013·Nature Reviews. Gastroenterology & Hepatology·Benjamin StrueckerIgor M Sauer
Sep 7, 2013·Cirugía española·Eugenia ParejaJose Mir
May 8, 2013·The Journal of Surgical Research·Mohamed S NoormohamedSteven A White
Aug 8, 2015·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Anil Dhawan
Oct 10, 2015·Tissue Engineering. Part C, Methods·Martin KlugeNathanael Raschzok
Sep 7, 2011·Gastroenterology Clinics of North America·Vinay Sundaram, Obaid S Shaikh
Jul 14, 2010·Gastroenterology·Laura ErkerMarkus Grompe
Oct 14, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yuta EnamiSanjeev Gupta
Sep 22, 2009·Journal of Internal Medicine·E FitzpatrickA Dhawan
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Aug 3, 2010·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Toshiyasu KawaharaNorman M Kneteman
Feb 1, 2011·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Eva TörökJoerg M Pollok
Jul 14, 2012·Journal of Internal Medicine·C JornsB G Ericzon
Mar 13, 2014·Annals of the New York Academy of Sciences·Celine Filippi, Anil Dhawan
Apr 29, 2015·Journal of Hepatology·Stuart J ForbesAnil Dhawan
Jul 19, 2015·Cryobiology·Maria-Louisa IzamisFrançois Berthiaume
Jul 30, 2010·Journal of Hepatology·Kyle A SoltysIra J Fox
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Sep 10, 2014·Cell Transplantation·Carl JornsEwa Ellis
Apr 8, 2014·Cell Transplantation·Roberto GramignoliStephen C Strom
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Nov 24, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jason M ZimmererGinny L Bumgardner

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