PMID: 6980952Jan 1, 1982Paper

Isolation of human and rat natural killer cells

Journal of Immunological Methods
T TimonenR B Herberman

Abstract

We describe a method for the purification of human and rat large granular lymphocytes (LGL), which are known to be the mediators of natural killer (NK) activity in these species. Plastic non-adherent and nylon wool passed blood mononuclear cells were separated into 7 fractions by discontinuous density gradient centrifugation on Percoll. Low density cells were highly enriched in LGL (up to 85% purity), whereas high density cells were typical small and medium sized lymphocytes devoid of NK activity. Human LGL could further be enriched by depleting high affinity sheep erythrocyte rosette-forming cells from the LGL-enriched Percoll fractions (resulting in up to greater than 90% purity). One critical variable in the separation technique was osmolarity, since the separation did not work optimally, if 290 mOsmoles/kg H2O in the Percoll solution was exceeded.

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Citations

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