Isolation of human basophils

Current Protocols in Immunology
D D Metcalfe

Abstract

Human basophils are normally the rarest of all circulating leukocytes and thus are among the most difficult of the blood elements to purify. In this unit, basophils are isolated from leukocyte populations, resulting in preparations of cells of which 5% to 50% are basophils and the rest are lymphocytes along with neutrophils and monocytes. This technique is advantageous because of its speed and because it minimizes basophil stimulation and histamine release.

References

Dec 4, 1987·Journal of Immunological Methods·J A WarnerD W MacGlashan
Jan 1, 1981·Journal of Immunological Methods·J J Pruzansky, R Patterson
Feb 1, 1984·Journal of Leukocyte Biology·E J LeonardA Skeel

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Citations

Feb 5, 2013·Scientific Reports·Meenu SharmaJagadeesh Bayry

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