Isolation of Human Basophils

Current Protocols in Immunology
John T Schroeder, Anja P Bieneman

Abstract

Isolating human basophils from blood has long been hampered by the fact that these granulocytes represent just 1% or less of the circulating leukocyte population. We describe herein laboratory protocols that have been refined over the past ∼25 years that now enable investigators to prepare basophils for use in a variety of assays to assess the in vitro biology of these immune cells, both in IgE -dependent and -independent responses.

References

Apr 25, 2008·Current Protocols in Immunology·W Strober
Jan 17, 2012·Journal of Immunological Methods·Pamela A Frischmeyer-Guerrerio, John T Schroeder
Nov 29, 2012·Current Allergy and Asthma Reports·Emily C McGowan, Sarbjit Saini
Feb 11, 2014·Current Protocols in Immunology·Florence M Hofman, Clive R Taylor
Jun 14, 2014·Chemical Immunology and Allergy·Gianni MaroneFrancescopaolo Granata

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Citations

Jun 28, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·John T Schroeder, Anja P Bieneman
Mar 14, 2021·The Journal of Allergy and Clinical Immunology·Kirti J JohalSarbjit S Saini

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