Isolation of human mesenchymal stem cells and their cultivation on the porous bone matrix

Journal of Visualized Experiments : JoVE
Nayeli Rodríguez-FuentesLuz E Alcantara-Quintana

Abstract

Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs bovine bone matrix Nukbone (NKB) as a scaffold. Thus, the application of MSCs in repair and tissue regeneration processes depends principally on the efficient implementation of the techniques for placing these cells in a host tissue. For this reason, the design of biomaterials and cellular scaffolds has gained importance in recent years because the topographical characteristics of the selected scaffold must ensure adhesion, proliferation and differentiation into the desired cell lineage in the microenvironment of the injured tissue. This option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) employs bovine bone matrix as a cellular scaffold and is an efficient culture technique because the cells respond to the topographic characteristics of the bovine bone matrix Nukbone (NKB), i.e., spreading on the surface, macroporous covering and colonizing the depth of the biomaterial, after the cell isolation process. We present the procedure for isol...Continue Reading

Citations

Mar 24, 2016·Stem Cells International·Denise SalzigPeter Czermak
Sep 22, 2016·Stem Cells International·Wioletta LechKrystyna Domanska-Janik
Mar 30, 2021·Bio-medical Materials and Engineering·Nayeli Rodríguez-FuentesJavier R Ambrosio

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Methods Mentioned

BETA
dissection
flow cytometry
Scanning Electron Microscopy

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