PMID: 6966664Jan 1, 1980Paper

Isolation of human T lymphocytes: comparison between nylon wool filtration and rosetting with neuraminidase (VCN) and 2-aminoethylisothiouronium bromide (AET)-treated sheep red blood cells (SRBC)

Journal of Immunological Methods
F IndiveriS Ferrone

Abstract

Isolation of human T lymphocytes by rosetting with SRBC, centrifugation on Ficoll-Hypaque gradients and lysis of SRBC, which reportedly affects the functional activities of lymphocytes, was compared to isolation of T lymphocytes by filtration through a nylon wool column. The two methods were similar in yields and purity of T lymphocytes. No selective loss of T cell subpopulations was found in T lymphocytes isolated by filtration through a nylon wool column as judged by the percentage of T gamma and T micron and the suppressor and helper activity in functional assays. Therefore, filtration through a nylon wool column is a useful method to purify T lymphocytes and has the advantage of being comparatively simple and quick to perform and of avoiding manipulations which may affect the functional activity of T lymphocytes.

References

Sep 22, 1977·Nature·M B OldstoneL Moretta
Jan 1, 1979·Journal of Immunological Methods·F IndiveriS Ferrone
Jan 1, 1977·Tissue Antigens·E GazitD Serr
Jan 1, 1975·European Journal of Immunology·M FerrariniM L Durante
Aug 1, 1975·European Journal of Immunology·L MorettaM C Mingari

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Citations

Jan 1, 1986·Archives of Dermatological Research·R E SchopfK Bork
Apr 1, 1983·Journal of Clinical Immunology·Z BrahmiR L Baehner
Sep 3, 1985·Journal of Immunological Methods·F IndiveriS Ferrone
May 31, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Zhang-Min YangLu-Sheng Si
Jan 22, 2000·The Journal of Clinical Investigation·M S LinL A Diaz
Feb 1, 1983·Human Immunology·F C GrumetE G Engleman
Nov 1, 1984·Journal of the Neurological Sciences·M E Fewster, B Kies
Apr 8, 2004·The Journal of Pediatrics·Andrew J CurrieDavid P Speert
Jan 1, 1983·Annals of the New York Academy of Sciences·A YachiM Kawaharada
Mar 14, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Mong-Shang LinJanet A Fairley
Oct 28, 2008·Molecular Immunology·Jillian E Wohler, Scott R Barnum
Oct 13, 2017·European Journal of Immunology·Andrea CossarizzaJakob Zimmermann
Sep 5, 2008·Current Protocols in Cytometry·J P McCoy
Jan 1, 1983·Annals of the New York Academy of Sciences·K ImaiA Yachi
Apr 25, 2008·Current Protocols in Immunology·M E Kanof
Jan 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Donald J DavidsonDavid P Speert
Dec 1, 1983·European Journal of Immunology·J Kagan, Y S Choi
Oct 22, 2019·European Journal of Immunology·Andrea CossarizzaArturo Zychlinsky
Mar 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dawn M E BowdishRobert E W Hancock
Feb 3, 2016·Current Protocols in Immunology·Marjorie E Kanof
May 26, 2016·Molecular Medicine Reports·Jian ShiHui-Min Wang

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