Isolation of integrin-based adhesion complexes

Current Protocols in Cell Biology
Matthew C JonesMartin J Humphries

Abstract

The integration of cells with their extracellular environment is facilitated by cell surface adhesion receptors, such as integrins, which play important roles in both normal development and the onset of pathologies. Engagement of integrins with their ligands in the extracellular matrix, or counter-receptors on other cells, initiates the intracellular assembly of a wide variety of proteins into adhesion complexes such as focal contacts, focal adhesions, and fibrillar adhesions. The proteins recruited to these complexes mediate bidirectional signaling across the plasma membrane, and, as such, help to coordinate and/or modulate the multitude of physical and chemical signals to which the cell is subjected. The protocols in this unit describe two approaches for the isolation or enrichment of proteins contained within integrin-associated adhesion complexes, together with their local plasma membrane/cytosolic environments, from cells in culture. In the first protocol, integrin-associated adhesion structures are affinity isolated using microbeads coated with extracellular ligands or antibodies. The second protocol describes the isolation of ventral membrane preparations that are enriched for adhesion complex structures. The protocols p...Continue Reading

References

Aug 3, 2007·Nature Cell Biology·Ronen Zaidel-BarBenjamin Geiger
Jan 30, 2008·Current Protocols in Cell Biology·M J Humphries
Jan 30, 2008·Current Protocols in Cell Biology·Dorothy A BeachamEdna Cukierman
Sep 10, 2009·Science Signaling·Jonathan D HumphriesMartin J Humphries
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Citations

Feb 3, 2016·The Journal of Cell Biology·Edward R HortonMartin J Humphries
Sep 5, 2015·Cancer Metastasis Reviews·D I CantorM S Baker
Jul 30, 2017·Seminars in Cell & Developmental Biology·Shumin Xia, Pakorn Kanchanawong
Nov 2, 2016·Journal of Cell Science·Edward R HortonMartin J Humphries
Jul 7, 2015·Proteomics. Clinical Applications·Jila N AjeianJonathan D Humphries
Oct 11, 2016·Proteomics·Aki Manninen, Markku Varjosalo
Mar 21, 2020·Frontiers in Cell and Developmental Biology·Mladen ParadžikAndreja Ambriović-Ristov
Dec 22, 2020·Frontiers in Physiology·Bernd Henning StumpfAna-Sunčana Smith
Jan 9, 2021·Cell Communication and Signaling : CCS·Ioanna AntoniadesParis A Skourides
Oct 30, 2016·The American Journal of Pathology·Sushmita GhatakBeate Eckes
May 19, 2021·Cellular Signalling·Yasaswi Gayatri Mishra, Bramanandam Manavathi
Oct 12, 2018·Science Translational Medicine·Eun Kyung SongTae Joo Park

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Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

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