PMID: 6411126Aug 29, 1983Paper

Isolation of lamellar bodies from rat granular pneumocytes in primary culture

Biochimica Et Biophysica Acta
A ChanderA B Fisher

Abstract

A lamellar body fraction was isolated from rat alveolar granular pneumocytes in primary culture by upward flotation on a discontinuous sucrose gradient and compared with a similar fraction isolated from lung homogenates. Lamellar bodies from granular pneumocytes were free of detectable contamination with either succinate dehydrogenase or NADPH-cytochrome c reductase. There was an enrichment of acid phosphatase activity, which, based on distribution of enzyme activity on the gradient, did not appear to be a contamination from other fractions. The lamellar body fraction of granular pneumocytes yielded approx. 1 microgram protein/10(6) cells with a phospholipid-to-protein ratio (mg/mg) of 9.6 +/- 0.4 (n = 7). Composition with respect to total phospholipids was 71.0% phosphatidylcholine (disaturated phosphatidylcholine, 45.2%), 8.4% phosphatidylglycerol and 12.8% phosphatidylethanolamine. Palmitic acid comprised 66% of the fatty acids in phosphatidylcholine and 34% of those in phosphatidylglycerol. The lamellar body fraction from granular pneumocytes was similar to that from whole lung with respect to phospholipid-to-protein ratio and phospholipid composition and showed only minor differences in fatty acid composition. Ultrastructu...Continue Reading

References

Sep 2, 1975·Biochemical and Biophysical Research Communications·H L SpitzerJ M Johnston
Feb 7, 1978·Biochemistry·G E Hook
Sep 27, 1976·Biochimica Et Biophysica Acta·M F Heath, W Jacobson
Feb 1, 1964·The Biochemical Journal·V P SkipskiM Barclay
Feb 21, 1972·Biochimica Et Biophysica Acta·B A Page-Roberts
Dec 16, 1974·Biochimica Et Biophysica Acta·J Goerke
Feb 1, 1982·Experimental Lung Research·M PostL M van Golde
Oct 1, 1980·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·A B FisherH Berman
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

❮ Previous
Next ❯

Citations

Jan 1, 1987·The International Journal of Biochemistry·M N Diez-BlancoM Llanillo
Aug 30, 2002·European Journal of Biochemistry·Lin LiuDavid M Terrian
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L G DobbsJ Nellenbogen
Jul 19, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Linghui ZhangShaohui Huang
Apr 13, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Aron B FisherSandra R Bates
Dec 9, 1998·American Journal of Respiratory Cell and Molecular Biology·K OsanaiD R Voelker
Mar 1, 1992·American Journal of Respiratory Cell and Molecular Biology·J B RubinsB F Dickey
Oct 1, 1992·American Journal of Respiratory Cell and Molecular Biology·M F BeersA B Fisher
Jun 26, 2008·BMC Cell Biology·Pengcheng WangLin Liu
Jun 19, 2007·FEBS Letters·Yoshihiro MatsumuraNobuya Inagaki
May 11, 2012·Cell Biology International·Pengcheng WangLin Liu
Jan 1, 1987·Differentiation; Research in Biological Diversity·B Zimmermann
Sep 14, 2011·Biochimica Et Biophysica Acta·Tudevdagva GerelsaikhanAvinash Chander
Oct 8, 2016·Developmental Biology·Jinwook ChoiJoo-Hyeon Lee
Mar 3, 2004·Free Radical Biology & Medicine·Vuokko L Kinnula, James D Crapo
Mar 10, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·A B Fisher, C Dodia
Nov 27, 2020·Molecular Cell·Navdar SeverTom A Rapoport
Nov 16, 1995·Biochimica Et Biophysica Acta·L LiuU J Zimmerman
Jan 13, 1987·Biochimica Et Biophysica Acta·J G NijssenH van den Bosch
Dec 4, 1985·Biochimica Et Biophysica Acta·A C de VriesL M van Golde
Jan 24, 1992·Biochimica Et Biophysica Acta·A Chander
Nov 5, 1991·Biochimica Et Biophysica Acta·A Chander, R D Wu
Jul 18, 2006·Biochimica Et Biophysica Acta·Avinash ChanderXiao-Liang Chen
Jun 17, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yaniv TomerSurafel Mulugeta
Sep 4, 2007·Biochimica Et Biophysica Acta·Avinash ChanderDevendra G Naidu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.