PMID: 1202026Dec 1, 1975Paper

Isolation of liver lysosomes by iron loading. Ultrastructural characterization

The Journal of Cell Biology
H GlaumannJ L Ericsson

Abstract

In summary, the data demonstrate that, by the use of repeated injections of an iron sorbitol complex, it is possible to isolate a fraction highly enriched in hydrolytic enzymes (60 times over the homogenate) and in well preserved lysosomes emanating almost entirely from liver parenchymal cells. The advantage of adding fixative to the bottom of the gradient and of using en bloc staining with uranyl acetate is also demonstrated.

References

Jul 1, 1972·The Histochemical Journal·D Hopwood
Nov 30, 1973·Biochimica Et Biophysica Acta·R HenningW Stoffel
Sep 9, 1967·Biochimica Et Biophysica Acta·R ColemanJ N Hawthorne
Jan 1, 1966·Annual Review of Physiology·C De Duve, R Wattiaux
Jul 25, 1956·The Journal of Biophysical and Biochemical Cytology·A B NOVIKOFFC DE DUVE
May 1, 1963·The Journal of Cell Biology·M G FARQUHAR, G E PALADE

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Citations

Jun 1, 1992·Environmental Research·A JohanssonP Camner
Jul 13, 2000·Journal of Biochemical and Biophysical Methods·H Pertoft
Aug 1, 1976·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W MrazH Jatzkewitz
Jan 1, 1984·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·R HultcrantzH Glaumann
Oct 1, 1976·FEBS Letters·S ReiterH Jatzkewitz
Aug 1, 1980·Experimental Cell Research·L MarzellaH Glaumann
Oct 15, 1977·Experimental Cell Research·D L KnookE C Sleyster
Oct 1, 1977·Experimental and Molecular Pathology·H Glaumann, J A Gustafsson
Jun 6, 2000·Biochimica Et Biophysica Acta·E DimitriouH Michelakakis
Feb 15, 1997·Microscopy Research and Technique·P SchneiderU Busch
Apr 27, 1977·Biochimica Et Biophysica Acta·J Carlstedt-DukeO Wrange
Oct 1, 1980·Experimental Cell Research·L MarzellaH Glaumann

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