PMID: 9523770Apr 2, 1998Paper

Isolation of liver nuclei that retain functional trans-membrane transport

Journal of Pharmacological and Toxicological Methods
Y F Ho, T M Guenthner

Abstract

We have developed a method for the rapid isolation of hepatocyte nuclei, which employs gentle homogenization and centrifugation conditions, and involves minimal processing time. The purified nuclei were morphologically unaltered when observed by light and electron microscopy. No significant contamination from cytoplasm or mitochondria was detected when assessed by marker enzymes. Membrane transport function, measured as ATP-dependent calcium uptake, was intact. This isolation method was devised to be applicable to studies that involve measurement of uptake and active transport of a variety of substances by the cell nucleus.

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Citations

Oct 7, 2009·Antioxidants & Redox Signaling·Montserrat MaríJosé C Fernández-Checa
Feb 4, 2012·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Pil-Hoon ParkShivendra D Shukla
Jun 15, 2011·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Katherine FormanJ A F Tresguerres
Apr 10, 2012·Seminars in Cell & Developmental Biology·Magdalena L Circu, Tak Yee Aw
Nov 21, 2009·Analytical Biochemistry·Takako NagataRaj Lakshman
Aug 13, 2011·Free Radical Research·Magdalena L Circu, Tak Yee Aw
Jan 5, 2010·Free Radical Biology & Medicine·Magdalena L Circu, Tak Yee Aw
Feb 20, 2018·Molecular and Cellular Biochemistry·Antônio da Silva NovaesMirian Aparecida Boim
Aug 6, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pil-Hoon ParkShivendra D Shukla
Aug 2, 2008·Free Radical Research·Magdalena L Circu, Tak Yee Aw
Jun 10, 2010·Free Radical Research·Jelena MarkovicFederico V Pallardó
Apr 20, 2019·Scientific Reports·Antônio da Silva NovaesMirian Aparecida Boim

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