Isolation of low density lipoprotein subfraction containing apolipoprotein B-like protein from Japanese eel Anguilla japonica plasma using dextran sulfate cellulose

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
S Ando, M Matsuzaki

Abstract

Japanese eel (Anguilla japonica) possessed a unique lipoprotein profile in their plasma, reflecting high utilization of lipids. Low density lipoprotein (LDL) fraction isolated at the densities from 1.006 to 1.085 g/ml comprised the heterogeneous components with molecular weight (Mr) 1200 K, 470 K, and 250 K. LDL subfraction with Mr 1200 K was completely adsorbed to dextran sulfate cellulose (DSC) column which had been developed for LDL apheresis treatment of the patients with familial hypercholesterolemia, while LDL subfractions with Mr 470 K and 250 K had no affinity for the DSC column. LDL subfractions with Mr 470 K and 250 K were floated and settled, respectively, by centrifuging the unbound fraction of DSC column at a density of 1.063 g/ml. LDL subfraction with Mr 1200 K possessed apolipoprotein (apo) B-like protein of Mr 230 K, while apo A-I- and A-II-like proteins of Mr 25 K and 14 K were the main components in LDL subfractions with Mr 470 K and 250 K. The presence of apo B-like protein seemed to be responsible for the adsorption of LDL subfraction with Mr 1200 K for the DSC column. LDL subfractions with Mr 470 K and 250 K seemed to belong to high density lipoprotein (HDL) with respect to molecular weights and apolipoprot...Continue Reading

References

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Citations

Jul 2, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·S Ando, K Yanagida
Mar 6, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·N ChimsungJ E Milley
Mar 23, 2000·Journal of Colloid and Interface Science·M MalmstenW G Wood
Mar 30, 2001·Biochimica Et Biophysica Acta·H KondoS Watabe

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