Isolation of lysozyme on chitosan

Biotechnology and Bioengineering
R A MuzzarelliR Rocchetti

Abstract

Lysozyme has been immobilized on chitosan, a polyaminosaccharide, without using any intermediate reagent. The best pH conditions for operating the chitosan columns have been determined and the best eluting agent was found to be a 2% solution of propylamine. The lysozyme activity was determined after reacting lysozyme with the product of glycolchitin and Remazol Brilliant Blue R. The recovery of lysozyme from chicken egg white yields lysozyme with 55% activity.

References

Apr 1, 1979·Biotechnology and Bioengineering·S Hirano, O Miura
Mar 1, 1982·Biotechnology and Bioengineering·Y NozawaF Higashide

Citations

Aug 1, 1975·Journal of Biochemistry·S KuramitsuK Nakashima
Oct 1, 1976·Biotechnology and Bioengineering·R A MuzzarelliR Rocchetti
Oct 5, 1975·Journal of Molecular Biology·C R BeddellS J Oatley
Oct 31, 1973·Journal of the American Chemical Society·L E SmithM A Raftery
Dec 15, 1973·Molecular and Cellular Biochemistry·J SkujiņśA D McLaren
Dec 1, 1974·Journal of Biochemistry·H Nakatani, K Hiromi
Apr 11, 1972·European Journal of Biochemistry·H B Jensen, K Kleppe

Related Concepts

Egg Whites
glycolchitin
Lysozyme Test
LYZ
Propylamines
Lysozyme
Muramidase
Remazol Brilliant Blue R
Leftose
Chitin

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