Isolation of mammary epithelial cells from three-dimensional mixed-cell spheroid co-culture.

Journal of Visualized Experiments : JoVE
Kun Xu, Rachel J Buchsbaum

Abstract

While enormous efforts have gone into identifying signaling pathways and molecules involved in normal and malignant cell behaviors(1-2), much of this work has been done using classical two-dimensional cell culture models, which allow for easy cell manipulation. It has become clear that intracellular signaling pathways are affected by extracellular forces, including dimensionality and cell surface tension(3-4). Multiple approaches have been taken to develop three-dimensional models that more accurately represent biologic tissue architecture(3). While these models incorporate multi-dimensionality and architectural stresses, study of the consequent effects on cells is less facile than in two-dimensional tissue culture due to the limitations of the models and the difficulty in extracting cells for subsequent analysis. The important role of the microenvironment around tumors in tumorigenesis and tumor behavior is becoming increasingly recognized(4). Tumor stroma is composed of multiple cell types and extracellular molecules. During tumor development there are bidirectional signals between tumor cells and stromal cells(5). Although some factors participating in tumor-stroma co-evolution have been identified, there is still a need ...Continue Reading

Citations

Dec 5, 2013·Journal of Visualized Experiments : JoVE·Golsa RazianMark Ungrin
Jan 30, 2016·Breast Cancer Research : BCR·Kun XuRachel J Buchsbaum
Apr 12, 2014·Journal of Cellular and Molecular Medicine·Aimei ZhongJiaming Sun
Jul 12, 2017·The Journal of Organic Chemistry·Alyssa H AntropowMohammad Movassaghi
Apr 20, 2017·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·H J RenL Ming
Nov 27, 2018·Journal of Mammary Gland Biology and Neoplasia·Nikitha K PallegarSherri L Christian
Jul 1, 2020·Frontiers in Bioengineering and Biotechnology·Patrícia Barros da SilvaCristina Carvalho Barrias

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