Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Journal of Visualized Experiments : JoVE
Florian S FruehMatthias W Laschke

Abstract

A functional microvascular network is of pivotal importance for the survival and integration of engineered tissue constructs. For this purpose, several angiogenic and prevascularization strategies have been established. However, most cell-based approaches include time-consuming in vitro steps for the formation of a microvascular network. Hence, they are not suitable for intraoperative one-step procedures. Adipose tissue-derived microvascular fragments (ad-MVF) represent promising vascularization units. They can be easily isolated from fat tissue and exhibit a functional microvessel morphology. Moreover, they rapidly reassemble into new microvascular networks after in vivo implantation. In addition, ad-MVF have been shown to induce lymphangiogenesis. Finally, they are a rich source of mesenchymal stem cells, which may further contribute to their high vascularization potential. In previous studies we have demonstrated the remarkable vascularization capacity of ad-MVF in engineered bone and skin substitutes. In the present study, we report on a standardized protocol for the enzymatic isolation of ad-MVF from murine fat tissue.

Citations

Oct 20, 2017·Journal of Tissue Engineering and Regenerative Medicine·Matthias W LaschkeMichael D Menger
Nov 24, 2018·Journal of Tissue Engineering and Regenerative Medicine·Matthias W LaschkeMichael D Menger
Mar 6, 2018·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Thomas SpäterMatthias W Laschke
Nov 25, 2020·Tissue Engineering. Part B, Reviews·Elana M MeijerCaroline Cheng
Nov 3, 2020·EMBO Molecular Medicine·Lisa NalbachEmmanuel Ampofo

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