PMID: 9541247Apr 16, 1998Paper

Isolation of murine cementoblasts: unique cells or uniquely-positioned osteoblasts?

European Journal of Oral Sciences
R L MacNeilM J Somerman

Abstract

While cementoblasts express a number of mineral-related proteins, including bone sialoprotein (BSP), osteopontin (OPN) and osteocalcin (OC), these proteins do not appear to be expressed by cells of the intermediate dental follicle/periodontal ligament (PDL). This information was utilized in an experimental strategy to isolate presumptive cementoblasts from the root surface of day 24 murine mandibular first molars. Using microscopic dissection techniques, molars were carefully extracted from their alveolar crypts and subjected to trypsin-collagenase digestion to remove adherent cells. Primary cultures were established and assayed for expression of proteins known to be expressed by cementoblasts at this timepoint in vivo (i.e. BSP, OPN, OC) and also an odontoblast-specific protein (i.e. DSP) to rule out contamination by pulpal cells. A subgroup of cells were found to express Type I collagen (89% of cells), BSP (46%), OPN (23%) and OC (30%); DSP was not detected within these cultures. We propose that cells within this heterogeneous population, which express this profile of osteogenic proteins, represent cementoblasts. The availability of a cementoblast cell line will make possible rigorous and controlled in vitro analysis of these...Continue Reading

Citations

Sep 7, 2013·Journal of Proteomics·Cristiane R SalmonFrancisco H Nociti
Jan 26, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·W J GrzesikM Yamauchi
Feb 10, 2006·Calcified Tissue International·B L FosterM J Somerman
Apr 8, 2011·Archives of Oral Biology·Julie Teresa MarchesanYvonne L Kapila
Jun 12, 2016·Biochemical and Biophysical Research Communications·Jing LiaoShujuan Zou
Jun 14, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·T YamamotoM Wakita
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·R T Franceschi
Mar 7, 2017·Journal of Periodontal Research·B L Foster
Apr 21, 2005·Journal of Dental Research·D D Bosshardt
Sep 1, 2007·Journal of Periodontal Research·L PhamS Tetradis
Oct 1, 2004·Journal of Periodontology·Fernanda BoabaidMartha J Somerman
Jan 20, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·F LézotA Berdal
Dec 14, 2017·International Journal of Oral Science·Nam C-N HuynhRuchanee S Ampornaramveth
Feb 24, 2000·Journal of Periodontal Research·M J SomermanW V Giannobile
Jan 6, 2021·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Irma AzraqMichael Wolf
Aug 2, 2003·Journal of Structural Biology·Marco Antonio Alvarez PérezHiginio Arzate

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