Isolation of mycobacterium-reactive CD1-restricted T cells from patients with human immunodeficiency virus infection

The Journal of Clinical Investigation
J GongP F Barnes

Abstract

Because CD1-restricted T cells lack CD4 but produce IFN-gamma in response to nonpeptide mycobacterial antigens, they could play a unique role in immunity to tuberculosis. We studied CD1-restricted T cells in the context of HIV infection by expanding CD4(-) T cell lines from 10 HIV-infected patients. Upon stimulation with Mycobacterium tuberculosis antigen or upon exposure to macrophages infected with M. tuberculosis, these T cell lines proliferated, produced IFN-gamma, and showed cytolytic T cell (CTL) activity against macrophages pulsed with mycobacterial antigen, findings consistent with a protective role against M. tuberculosis. Anti-CD1b antibodies abrogated T cell proliferation, IFN-gamma production, and CTL activity, demonstrating that these T cells are CD1 restricted. IFN-gamma production in response to M. tuberculosis was enhanced by antitransforming growth factor-beta in 8/10 lines, and by IL-15 in 2/10 lines. IFN-gamma production was augmented in a nonantigen-specific manner by IL-12 in 4/8 lines. When live HIV was cocultured with CD1-restricted T cell lines, p24 antigen and proviral DNA were not detected, indicating that the T cells were not infectable with HIV. Vaccination strategies aimed at activation and expansio...Continue Reading

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Citations

Sep 2, 1998·Current Opinion in Immunology·S Stenger, R L Modlin
Feb 27, 1999·Current Opinion in Microbiology·S Stenger, R L Modlin
Aug 18, 2000·Immunology and Cell Biology·S M Smith, H M Dockrell
Jun 8, 1999·Annual Review of Immunology·S A Porcelli, R L Modlin
Mar 23, 2004·Annual Review of Immunology·Manfred Brigl, Michael B Brenner
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Nov 25, 2006·The Lancet Infectious Diseases·Rajko Reljic, Juraj Ivanyi
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Sep 11, 2010·Veterinary Immunology and Immunopathology·Sharla M PetersMichael J Myers
Jan 10, 2018·Frontiers in Immunology·Ulrich E SchaibleTobias Dallenga
Aug 23, 2001·Scandinavian Journal of Infectious Diseases·S Stenger

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