Isolation of N2 -fixing rhizobacteria from Lolium perenne and evaluating their plant growth promoting traits

Journal of Basic Microbiology
Antonio Castellano-HinojosaEulogio J Bedmar

Abstract

Twenty one dinitrogen (N2 )-fixing bacteria were isolated from the rhizosphere of Lolium perenne grown for more than 10 years without N-fertilization. The nearly complete sequence of the 16S rRNA gene of each strain and pairwise alignments among globally aligned sequences of the 16S rRNA genes clustered them into nine different groups. Out of the 21 strains, 11 were members of genus Bacillus, 3 belonged to each one of genera Paenibacillus and Pseudoxanthomonas, and the remaining 2 strains to each one of genera Burkholderia and Staphylococcus, respectively. A representative strain from each group contained the nifH gene and fixed atmospheric N2 as determined by the acetylene-dependent ethylene production assay (acetylene reduction activity, ARA). The nine selected strains were also examined to behave as plant growth promoting bacteria (PGPRs) including their ability to act as a biocontrol agent. The nine representative strains produced indol acetic acid (IAA) and solubilized calcium triphosphate, five of them, strains C2, C3, C12, C15, and C16, had ACC deaminase activity, and strains C2, C3, C4, C12, C16, and C17 produced siderophores. Strains C13, C16, and C17 had the capability to control growth of the pathogen Fusarium oxyspo...Continue Reading

References

Jan 1, 1994·Methods in Enzymology·S M Payne
May 26, 2001·Current Microbiology·S Mehta, C S Nautiyal
Apr 19, 2003·Physiologia Plantarum·Donna M. Penrose, Bernard R. Glick
Jun 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Katherina J KechrisAlexander N Glazer
Aug 12, 2009·Ecotoxicology and Environmental Safety·Beatriz MorenoEmilio Benitez
May 18, 2010·Applied and Environmental Microbiology·C TalbiE J Bedmar
Dec 6, 2011·International Journal of Systematic and Evolutionary Microbiology·Ok-Sun KimJongsik Chun
Feb 16, 2013·Genetics and Molecular Biology·Anelise BeneduziLuciane M P Passaglia
Feb 4, 2014·Microbiological Research·Nádia CastanheiraPaula Fareleira

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Citations

Jan 26, 2018·Journal of Applied Microbiology·A Castellano-HinojosaN Santillana
Feb 5, 2021·Frontiers in Plant Science·Luis Gabriel Cueva-YesquénFabiana Fantinatti-Garboggini

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